An automatic image analysis system for RLGS films

被引：0
作者：
Sugahara Y. [1 ,2 ,3 ,4 ]
Akiyoshi S. [1 ,4 ]
Okazaki Y. [1 ,4 ]
Hayashizaki Y. [1 ,3 ,4 ]
Tanihata I. [2 ]
机构：
[1] Genome Science Laboratory, RIKEN Tsukuba Life Science Center, Tsukuba, Ibaraki 305, 3-1-1, Koyadai
[2] Linac Laboratory, RIKEN, Wako, Saitama 351-01, 2-1, Hirosawa
[3] CREST, JST, Saitama 322, 4-1-8, Honcho, Kawaguchi
来源：
Mammalian Genome | 1998年 / 9卷 / 8期
关键词：
Mapping System; Genetic Data; Binary Image; Visual Observation; Initial Screening;
D O I：
10.1007/s003359900837
中图分类号：
学科分类号：
摘要：
The RLGS (Restriction Landmark Genome Scanning) method was originally developed as a powerful method for enabling viewing of thousands of restriction landmarks. It offers a tool for obtaining information about genetic loci, with a single RLGS profile displaying approximately 2000 restriction landmarks as spots. One of the most useful applications is RLGS spot mapping, which allows the efficient, low-cost construction of the genetic map of any organism. However, analyses of the profiles depend mainly on human visual observation and are tedious and laborious. Although several commercially available image analyzing systems for profile comparison have been examined, they cannot be used for the RLGS spot mapping system owing to the background characteristics of the RLGS profiles, unsatisfactory rates of correspondence, and inefficient correction of informative genetic data. We therefore developed a novel automatic image analysis system for RLGS spot mapping, using an original algorithm based on the binary image transferred from the original RLGS profile. This system was employed for identifying non- polymorphic and parental strain-specific polymorphic spots of the F1 mouse profile and yielded efficient initial screening of RLGS profiles.
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页码：643 / 651
页数：8
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