Role of protein kinase C during insulin mediated skeletal muscle cell spreading

被引:0
作者
Hira Lal Goel
Chinmoy Sankar Dey
机构
[1] National Institute of Pharmaceutical Education and Research,Signal Transduction Research Laboratory, Department of Biotechnology
来源
Journal of Muscle Research & Cell Motility | 2002年 / 23卷
关键词
Focal Adhesion Kinase; Focal Adhesion; Tyrosine Phosphorylation; Cell Spreading; Paxillin;
D O I
暂无
中图分类号
学科分类号
摘要
Protein kinase C (PKC) is known to play important roles in integrin mediated cell spreading. This study investigated the role of PKC during insulin mediated muscle cell spreading, which was independent of integrin α5. We found that PKC-α becomes active and localise to membrane during insulin mediated cell spreading. We also found that PKC activation is essential for cell spreading stimulated by insulin and this activation enhances the cell spreading. PKC activation increased the tyrosine phosphorylation of focal adhesion kinase (FAK) and paxillin as well as tyrosine kinase activity of FAK. We also observed that PKC activation enhanced the FAK associated PI 3-kinase activity and also increased the activation of ERK-1/-2. Moreover, the effect of PKC activation on insulin mediated cell spreading as well as tyrosine phosphorylation of FAK and paxillin depends upon integrity of actin cytoskeleton. Thus, PKC is an important signaling protein during insulin mediated muscle cell spreading.
引用
收藏
页码:269 / 277
页数:8
相关论文
共 187 条
[1]  
Bandyopadhyay G(1997)Activation of protein kinase C (α, β, and ς,) by insulin in 3T3/L1 cells J Biol Chem 274 2551-2558
[2]  
Standaert ML(1994)The RhoA-dependent assembly of focal adhesions in swiss 3T3 cells is associated with increased tyrosine phosphorylation and the recruitment of both pp125FAK and protein kinase delta to focal adhesions J Cell Sci 107 2033-2045
[3]  
Zhao L(1996)Focal adhesions, contractility, and signalling Ann Rev Cell Dev Biol 12 463-519
[4]  
Yu B(1994)Integrin mediated cell adhesion activates mitogen-activated protein kinases J Biol Chem 269 26,602-26,605
[5]  
Avignon A(1995)Integrins and signal transduction pathways: the road taken Science 268 233-239
[6]  
Galloway L(1997)Dissection of pathways implicated in integrin-mediated actin cytoskeleton assembly J Biol Chem 272 21,726-21,734
[7]  
Karnam P(1996)Integrin α J Biol Chem 271 11,016-11,022
[8]  
Moscat J(1999)β J Biol Chem 274 32,486-32,492
[9]  
Farese RV(1999)-dependent serine phosphorylation of paxillin in cultured human macrophages adherent to vitronectin J Cell Biol 144 1337-1347
[10]  
Barry ST(1994)Integrin mediated muscle cell spreading-the role of protein kinase C in outside-in and inside-out signaling and evidence of integrin cross-talk J Cell Biol 124 619-626