Gene Cloning and Characterization of a Novel Highly Organic Solvent Tolerant Lipase from Proteus sp. SW1 and its Application for Biodiesel Production

被引：0
作者：
Wirongrong Whangsuk
Pareenart Sungkeeree
Sirinthra Thiengmag
Jarunee Kerdwong
Ratiboot Sallabhan
Skorn Mongkolsuk
Suvit Loprasert
机构：
[1] Chulabhorn Research Institute,Laboratory of Biotechnology
[2] Chulabhorn Graduate Institute,Applied Biological Sciences Program
[3] CHE,Center of Excellence on Environmental Health and Toxicology
[4] Ministry of Education,undefined
来源：
Molecular Biotechnology | 2013年 / 53卷
关键词：
Glycerol-ester hydrolase; Triacylglycerol acylhydrolase; Alkaline lipase; Biodiesel; Transesterification; Biofuel; Alcohol resistant;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Proteus sp. SW1 was found to produce an extracellular solvent tolerant lipase. The gene, lipA, encoding a bacterial lipase, was cloned from total Proteus sp. SW1 DNA. lipA was predicted to encode a 287 amino acid protein of 31.2 kDa belonging to the Group I proteobacterial lipases. Purified His-tagged LipA exhibited optimal activity at pH 10.0 and 55°C. It was highly stable in organic solvents retaining 112% of its activity in 100% isopropanol after 24 h, and exhibited more than 200% of its initial activity upon exposure to 60% acetone, ethanol, and hexane for 18 h. Biodiesel synthesis reactions, using a single step addition of 13% an acyl acceptor ethanol, showed that LipA was highly effective at converting palm oil into biodiesel.
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页码：55 / 62
页数：7
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