Beta-Glucosidase Production Optimization from Newly Isolated Aspergillus tubingensis IMMIS2 Using Taguchi Statistical Design

Fungal production of beta-glucosidase was optimized by Taguchi statistical method using corn stover as substrate. Taguchi statistical design depicted the highest glucosidase activity (116 μg mL−1 min−1) by Aspergillus tubingensis IMMIS2 using 5 g of 80-mm mesh-sized substrate with 50% moisture contents, 0.1 g of urea, 0.2 g of KCl, 0.3 g of CaCl2, 0.01 g of MgSO4 and 3 mL of the fungal culture as inoculum. Main effect plot for SN ratio exposed that some parameters (corn stover as substrate, corn stover particle size, urea level and CaCl2) had significant effect on cellulase production as compared to some other parameters (inoculum size, KCl and MgSO4 levels). Our findings of the present study will be helpful for cost-effective and optimum production of commercially important enzymes using agro-industrial wastes as growth substrates.