Effect of cell plating density and extracellular matrix protein on cell growth of epithelial rests of Malassez in vitro

被引：10

作者：

Nishimura M. ^{[1
]}

Abiko Y. ^{[1
]}

Mitamura J. ^{[1
]}

Kaku T. ^{[1
]}

机构：

[1] Department of Oral Pathology, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu, Ishikari

来源：

Medical Electron Microscopy | 1999年 / 32卷 / 2期

关键词：

Apoptosis; Cell-cell interaction; Epithelial rest of Malassez; Extracellular matrix; In vitro;

D O I：

10.1007/s007950050019

中图分类号：

学科分类号：

摘要：

The present study investigated how cell plating density and extracellular matrix protein influence cell survival of epithelial rests of Malassez (ERM) in vitro. ERM cells were plated on culture dishes coated with laminin (LM), type IV collagen (type IV), or fibronectin (FN), or on noncoated dishes, (Non) at a cell density of 2 × 104-1 × 105/ml in a nonserum culture medium. XTT assays were performed to calculate the number of cells attached on the substrata after 6, 24, 48, and 72 h. Mean cell numbers were calculated, and significant differences were determined using ANOVA. When epithelial cells were cultured on various matrices at a cell density of less than 2 × 104/ml, the cells did not grow and then fell into apoptotic cell death, which was confirmed by transmission electron microscopy. The cell number was significantly increased in cells plated on FN compared to those on Non at a cell density of 4 × 104/ml-8 × 10 4/ml. These results suggest that both extracellular matrix proteins and cell-cell interactions may contribute to prevent apoptosis of ERM in vitro. Cell-cell interactions may be a more important factor than exogenous extracellular matrix proteins for the survival of ERM cells in vitro. © The Clinical Electron Microscopy Society of Japan 1999.

引用

页码：127 / 132

页数：5

共 22 条

[1] Inoue T., Enokiya Y., Hashimoto S., Fukumashi K., Shimono M., An experimental study on cell interaction between fibroblasts of periodontal ligament and cells from Malassez epithelial rests co-cultured in vitro, Jpn J Oral Biol, 37, pp. 356-364, (1995)
[2] Wessenlink P.R., Beertsen W., The prevalence and distribution of rests of Malassez in the mouse molar and their possible role in repair and maintenance of the periodontal ligament, Arch Oral Biol, 38, pp. 399-403, (1993)
[3] Ishizaki Y., Voyvodic J.T., Burne J.F., Raff M.C., Control of lens epithelial cell survivals, J Cell Biol, 121, pp. 899-908, (1993)
[4] Bates R.C., Buret A., Van Helden D.F., Horton M.A., Burns G.F., Apoptosis induced by inhibition of intercellular contact, J Cell Biol, 125, pp. 403-415, (1994)
[5] Boudreau N., Sympsn C.J., Werb Z., Bissell M.J., Suppression of ICE and apoptosis in mammary epithelial cells by extracellular matrix, Science, 267, pp. 891-893, (1995)
[6] Aoshiba K., Rennard S.I., Spurzen J.R., Cell-matrix and cell-cell interactions modulate apoptosis of branchial epithelial cells, Am J Physiol, 272, (1997)
[7] Merio G.R., Cella N., Hynes N.E., Apoptosis is accompanied by changes in bcl-2 and inhibited by specific extracellular matrix proteins in mammary epithelial cells, Cell Growth Differ, 8, pp. 251-260, (1997)
[8] Ilic D., Almeida E.A.C., Schlaepfer D.D., Dazin P., Aizawa S., Damsky C.H., Extracellular matrix survival signals transduced by focal adhesion kinase suppress p53-mediated apoptosis, J Cell Biol, 143, pp. 547-560, (1998)
[9] Howell S.J., Doane K.J., Type VI collagen increase cell survival and prevents anti-β1 integrin-mediated apoptosis, Exp Cell Res, 241, pp. 230-241, (1998)
[10] Ferrelly N., Lee Y.-J., Oliver J., Dive C., Streuli C.H., Extracellular matrix regulates apoptosis in mammary epithelium through a control on insulin signaling, J Cell Biol, 144, pp. 1337-1347, (1999)

← 1 2 3 →