Preparation of mycobacterial antigens fused with a cellulose-binding domain with the aim of developing a subunit antituberculous vaccine

被引：0
作者：
O. V. Sergienko
E. I. Aksenova
A. M. Lyashchuk
Z. M. Galushkina
N. N. Poletaeva
N. E. Sharapova
A. S. Semikhin
A. P. Kotnova
A. M. Veselov
V. N. Bashkirov
N. L. Kulikova
V. S. Khlebnikov
T. K. Kondrat’eva
A. S. Karyagina
A. S. Apt
V. G. Lunin
A. L. Gintsburg
机构：
[1] Ministry of Health and Social Development of the Russian Federation,Gamaleya Scientific Research Institute of Epidemiology and Microbiology
[2] Russian Academy of Agricultural Sciences,Institute of Agricultural Biotechnology
[3] Lomonosov Moscow State University,Faculty of Bioengineering and Bioinformatics
[4] Institute of Immunological Engineering,Central Scientific Research Institute of Tuberculosis
[5] Russian Academy of Medical Sciences,Belozerskii Institute of Physical and Chemical Biology
[6] Moscow State University,undefined
来源：
Molecular Genetics, Microbiology and Virology | 2012年 / 27卷
关键词：
tuberculosis; antigens; cellulose binding domain; subunit vaccines;
D O I：
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学科分类号：
摘要：
The genes of ESAT-6, CFP-10 and Ag85A secreted antigens of Mycobacterium tuberculosis were cloned in Escherichia coli. Fusion proteins Esat6-CBD, Ag85A-CBD and CFP10-CBD with thermostable cellulose binding domain (CBD) of Anaerocellum thermophilum providing high affinity to cellulose were constructed. Content of the fused proteins in overproducing strains was 20% of total protein for CFP10-CBD and 15% for both Esat6-CBD and Ag85A-CBD. Immobilization of the fusion proteins on cellulose is a one-stage process that provides simultaneous antigen purification and absorption on cellulose. The proteins retain their antigenic properties and can be used as the components for subunit tuberculosis vaccine production or in diagnostic kits.
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页码：16 / 21
页数：5
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