Discrimination of Infectious Hepatitis A Viruses by Propidium Monoazide Real-Time RT-PCR

被引：0

作者：

Gloria Sánchez

Patricia Elizaquível

Rosa Aznar

机构：

[1] Instituto de Agroquímica y Tecnología de Alimentos,Departamento de Biotecnología

[2] University of Valencia,Department of Microbiology and Ecology

[3] Institute of Agrochemistry and Food Technology (IATA),undefined

[4] Spanish Council for Scientific Research (CSIC),undefined

来源：

Food and Environmental Virology | 2012年 / 4卷

关键词：

Hepatitis A virus; Quantitative PCR; Infectivity; Propidium monoazide;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The discrimination of infectious and inactivated viruses remains a key obstacle when using quantitative RT-PCR (RT-qPCR) to quantify enteric viruses. In this study, propidium monoazide (PMA) and RNase pretreatments were evaluated for the detection and quantification of infectious hepatitis A virus (HAV). For thermally inactivated HAV, PMA treatment was more effective than RNase treatment for differentiating infectious and inactivated viruses, with HAV titers reduced by more than 2.4 log10 units. Results showed that combining 50 μM of PMA and RT-qPCR selectively quantify infectious HAV in media suspensions. Therefore, PMA treatment previous to RT-qPCR detection is a promising alternative to assess HAV infectivity.

引用

页码：21 / 25

页数：4

共 50 条

[41] UV inactivation and resistance of rotavirus evaluated by integrated cell culture and real-time RT-PCR assay
Li, Dan
Gu, April Z.
He, Miao
Shi, Han-Chang
Yang, Wan
WATER RESEARCH, 2009, 43 (13) : 3261 - 3269
[42] A Comparative Study for the Efficient Detection of Norovirus from Drinking Water by RT-PCR and Real-Time PCR
Anbazhagi, S.
Kamatchiammal, S.
WATER AIR AND SOIL POLLUTION, 2010, 213 (1-4): : 71 - 84
[43] Quantification of Viable Cells of Pseudomonas syringae pv. tomato in Tomato Seed Using Propidium Monoazide and a Real-Time PCR Assay
Chai, A-li
Ben, Hai-yan
Guo, Wei-tao
Shi, Yan-xia
Xie, Xue-wen
Li, Lei
Li, Bao-ju
PLANT DISEASE, 2020, 104 (08) : 2225 - 2232
[44] Analysis of viable vs. dead Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis using selective quantitative real-time PCR with propidium monoazide
Sanchez, M. C.
Marin, M. J.
Figuero, E.
Llama-Palacios, A.
Herrera, D.
Sanz, M.
JOURNAL OF PERIODONTAL RESEARCH, 2013, 48 (02) : 213 - 220
[45] Development and Evaluation of a Fluorogenic Real-Time RT-PCR for the Detection of Dengue 3 Virus
Tan, Irene L.
Dimamay, Mark Pierre S.
Buerano, Corazon C.
Alfon, Jhoe Anthony R.
Tanig, Carol Z.
Matias, Ronald R.
Natividad, Filipinas F.
JOURNAL OF MEDICAL VIROLOGY, 2010, 82 (12) : 2053 - 2063
[46] Quantification of melanoma micrometastases in sentinel lymph nodes using real-time RT-PCR
Giese, T
Engstner, M
Mansmann, U
Hartschuh, W
Arden, B
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2005, 124 (03) : 633 - 637
[47] Quantitation of hepatitis A virus and enterovirus levels in the lagoon canals and Lido beach of Venice, Italy, using real-time RT-PCR
Rose, Michael A.
Dhar, Arun K.
Brooks, Hilary A.
Zecchini, Fulvio
Gersberg, Richard M.
WATER RESEARCH, 2006, 40 (12) : 2387 - 2396
[48] Performance of Two Real-Time RT-PCR Assays for the Quantification of GI and GII Noroviruses and Hepatitis A Virus in Environmental Water Samples
De Keuckelaere, Ann
Stals, Ambroos
Baert, Leen
Uyttendaele, Mieke
FOOD ANALYTICAL METHODS, 2013, 6 (04) : 1016 - 1023
[49] Performance of Two Real-Time RT-PCR Assays for the Quantification of GI and GII Noroviruses and Hepatitis A Virus in Environmental Water Samples
Ann De Keuckelaere
Ambroos Stals
Leen Baert
Mieke Uyttendaele
Food Analytical Methods, 2013, 6 : 1016 - 1023
[50] Propidium Monoazide Coupled with PCR Predicts Infectivity of Enteric Viruses in Swine Manure and Biofertilized Soil
Fongaro, Gislaine
Hernandez, Marta
Cruz Garcia-Gonzalez, Maria
Monte Barardi, Celia Regina
Rodriguez-Lazaro, David
FOOD AND ENVIRONMENTAL VIROLOGY, 2016, 8 (01) : 79 - 85

← 1 2 3 4 5 →