Small GTP-Binding Protein Gene Is Associated with QTL for Submergence Tolerance in Rice

被引:0
|
作者
V. Ruanjaichon
D. Sangsrakru
W. Kamolsukyunyong
M. Siangliw
T. Toojinda
S. Tragoonrung
A. Vanavichit
机构
[1] Kasetsart University,Rice Gene Discovery Unit, National Center for Genetic Engineering and Biotechnology
来源
Russian Journal of Plant Physiology | 2004年 / 51卷
关键词
Oryza sativa; Ras-related GTP-binding protein; single strand conformation polymorphism; submergence stress;
D O I
暂无
中图分类号
学科分类号
摘要
Small GTP-binding proteins play critical roles in signal transduction in mammalian and plant systems. In this study, sequence variation of a small GTP-binding protein identified in the subgenomic region was analyzed. The major quantitative trait locus (QTL) controlling submergence tolerance on the 6.5-cM region of chromosome 9 was previously mapped, sequenced, and annotated. One of the most interesting candidate genes located in this QTL was a 5.2-kb sequence, which included a coding sequence consisting of two exons and a promoter. The deduced amino acid sequence corresponded to a 24.8 kD protein consisting of 226 amino acids, with 98% identity to RGP1, a small GTP-binding protein involved in a signal pathway responding to hormones, such as cytokinin and ethylene. According to the amino acid sequence, a putative small G-protein was classified as a small Ras-related GTP-binding protein. DNA gel blot analysis showed that the putative gene encoding the Ras-related GTP-binding protein was present as a single copy in the rice genome. Comparison of genomic sequences from several rice cultivars tolerant to submergence identified single nucleotide polymorphisms located in the TATA box of the Ras promoter region. Linkage analysis showed that the putative gene for GTP-binding protein was tightly linked to the peak of the QTL previously mapped on the long arm of chromosome 9. The single strand conformation polymorphism of the putative GTP-binding protein gene can be used for allele discrimination and marker assisted selection for tolerance to flash flooding.
引用
收藏
页码:648 / 657
页数:9
相关论文
共 50 条
  • [41] The gene for a small Rab7-related GTP-binding protein is expressed in apomictic and sexual buffelgrass
    Gustine, DL
    Hulce, DA
    Moyer, BG
    Sherwood, RT
    PLANT PHYSIOLOGY, 1996, 111 (02) : 535 - 535
  • [42] Rice gibberellin-insensitive dwarf mutant gene Dwarf 1 encodes the α-subunit of GTP-binding protein
    Ashikari, M
    Wu, JZ
    Yano, M
    Sasaki, T
    Yoshimura, A
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (18) : 10284 - 10289
  • [43] Small GTP-binding Proteins and their Functions in Plants
    Qing-Hu Ma
    Journal of Plant Growth Regulation, 2007, 26 : 369 - 388
  • [44] THE RAS SUPERFAMILY OF SMALL GTP-BINDING PROTEINS
    DOWNWARD, J
    TRENDS IN BIOCHEMICAL SCIENCES, 1990, 15 (12) : 469 - 472
  • [45] Small GTP-binding proteins and their functions in plants
    Ma, Qing-Hu
    JOURNAL OF PLANT GROWTH REGULATION, 2007, 26 (04) : 369 - 388
  • [46] Regulation of small GTP-binding proteins by insulin
    Welsh, GI
    Hers, I
    Wherlock, M
    Tavaré, JM
    BIOCHEMICAL SOCIETY TRANSACTIONS, 2006, 34 : 209 - 212
  • [47] THE CELLULAR FUNCTIONS OF SMALL GTP-BINDING PROTEINS
    HALL, A
    SCIENCE, 1990, 249 (4969) : 635 - 640
  • [48] SMALL GTP-BINDING PROTEINS IN VESICULAR TRANSPORT
    BALCH, WE
    TRENDS IN BIOCHEMICAL SCIENCES, 1990, 15 (12) : 473 - 477
  • [49] Small GTP-binding proteins in Plasmodium falciparum
    Jambou, R
    Zahraoui, A
    Olofsson, B
    Tavitian, A
    Jaureguiberry, G
    BIOLOGY OF THE CELL, 1996, 88 (03) : 113 - 121
  • [50] PURIFICATION OF A GTP-BINDING PROTEIN LOCALIZED IN MITOCHONDRIA
    TAKEDA, S
    SAGARA, Y
    KITA, K
    NATORI, S
    SEKIMIZU, K
    JOURNAL OF BIOCHEMISTRY, 1993, 114 (05): : 684 - 690