Identification of seeds ofPinus species by Microsatellite Markers

The 276 pair-primers (nuclear and chloroplast microsatellite) developed from seven species of Pinaceae were selected and identified for cross-species transferability to tenPinus species (P. massoniana, P. kesiya, P. tabulaeformis, P. densiflora, P. thunbergii, P. caribaea, P. taeda, P. yunnanensis, P. densata, P. sylvestris) belonging to Sect.Pinus by BSA (bulked segregate analysis) method. The results showed that 23 of 276 (8.0%) markers were successful to have amplification product in ten species, and 5 of 23 (21.7%) were polymorphic cross species and lack of polymorphic within species. Eight of 10Pinus species were identified by using single primer, two and more combination of primers, but there were still no effective SSR primers for identifying other 2 species (P. kesiya andP. densata).