Melanin Nanoparticles (MNPs) provide protection against whole-body ɣ-irradiation in mice via restoration of hematopoietic tissues

被引：0
作者：
Monira M. Rageh
Reem H. EL-Gebaly
H. Abou-Shady
Doaa G. Amin
机构：
[1] Cairo University,Department of Biophysics, Faculty of Science
[2] Cairo University,Department of Physics, Faculty of Science
[3] Center of Nuclear Studies and Peaceful Application,undefined
来源：
Molecular and Cellular Biochemistry | 2015年 / 399卷
关键词：
Melanin nanoparticles; Radioprotection; ɤ-irradiation; DNA damage;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
During radiotherapy, ionizing irradiation interacts with biological systems to produce free radicals, which attack various cellular components. The hematopoietic system is easily recognized to be radiosensitive and its damage may be severe. Melanin nanoparticles (MNPs) act as free radical scavengers prepared by polymerization of dopamine. In this study, a total of 110 male BALB/C mice were divided into five equal groups. Each group contained 22 mice. Mice of group A did not receive MNPs or irradiation (control group), group B was injected intraperitoneally (i.p.) with 50 mg/kg MNPs. Mice of group C and D were exposed to a dose of 7 Gy ɣ-irradiation and injected with the same dose of MNPs as in group B either 30 min pre- or post-irradiation, and group E was exposed to a dose of 7 Gy ɣ-irradiation only. The impact of MNPs on peripheral blood, spleen, and DNA damage induced by irradiation was evaluated by blood count, histopathology of the spleen, and comet assay for the DNA in the bone marrow at 1, 4, 8, and 12 days post-irradiation. Results of group E compared with control group (A) showed a significant depression in complete blood count. Additionally, histopathological observation showed the absence of megakaryocytes with delayed time post-irradiation, deposition of eosinophilic protein of their spleen appeared, as well as a remarkable decrease in spleen size was observed. Moreover, ɣ-irradiation-induced DNA damage as can be inferred from a significant increase by about 5–10 folds in all comet parameters (% of DNA, tail length, tail moment, and olive moment) in the DNA of the bone marrow. In contrast, pre-post treatment with MNPs protected hematopoietic tissues against radiation damage, and therefore, enhanced the survival of mice with 40 % in groups (C&D) compared with 10 % to group (E) till 30 days post-irradiation. In conclusion, these results demonstrated that synthetic MNPs provide significant radioprotection to the hematopoietic tissues.
引用
收藏
页码：59 / 69
页数：10
相关论文
共 174 条
[1]  
Shirazi A(2013)Radio-protective effects of melatonin against irradiation-induced oxidative damage in rat peripheral blood Phys Med 29 65-74
[2]  
Mihandoost E(2008)Protective effect of N-acetylcysteine against radiation induced DNA damage and hepatic toxicity in rats Biochem Pharmacol 75 773-780
[3]  
Mohseni M(2005)Radiation and the microenvironment-tumorigenesis and therapy Nat Rev Cancer 5 867-875
[4]  
Ghazi-Khansari M(2012)Antioxidant activities of Ganoderma lucidum polysaccharides and their role on DNA damage in mice induced by cobalt-60 gamma-irradiation Food Chem Toxicol 50 303-309
[5]  
Rabie Mahdavi S(2008)DNA damage induced by the direct effect of radiation Radiat Phys Chem 77 1280-1285
[6]  
Mansour HH(2012)Structural characterization and radioprotection of bone marrow hematopoiesis of two novel polysaccharides from the root of Fitoterapia 83 712-1720
[7]  
Hafez HF(2002) (Oliv.) Diels Exp Hematol 30 513-528
[8]  
Fahmy NM(2005)Hematologic consequences of exposure to ionizing radiation Exp Mol Pathol 78 156-169
[9]  
Hanafi N(2007)Role of cytokines and growth factors in radioprotection Drug Discov Today 12 794-805
[10]  
Barcellos-Hoff MH(2005)Trends in the development of radioprotective agents Mutagenesis 20 37-43
12345678910