Short-term hypoxia induces a selective death of GABAergic neurons

被引：10

作者：

Turovskaya M.V. ^{[1
]}

Turovsky E.A. ^{[1
]}

Kononov A.V. ^{[1
]}

Zinchenko V.P. ^{[1
]}

机构：

[1] Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Oblast, 142290, ul. Institutskaya

来源：

Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology | 2014年 / 8卷 / 1期

关键词：

GABAergic neurons; hyperexcitability; hypoxia; NMDA receptors; selective death;

D O I：

10.1134/S199074781305019X

中图分类号：

学科分类号：

摘要：

It is known that brief episodes of hypoxia protect neurons from death caused by global ischemia and hypoxia (hypoxic preconditioning). At the same time, brief hypoxia may cause a phenomenon of posthypoxic hyperexcitability during reoxygenation, which can lead to the death of separate neurons due to their individual differences. In this work we compare the effects of short-term hypoxia on the initiation of preconditioning and posthypoxic hyperexcitability in two populations of neurons: inhibitory GABAergic neurons and excitatory glutamatergic neurons. Preconditioning effect was evaluated according to the suppression of the NMDA-receptor activity. The phenomenon of posthypoxic hyperexcitability was estimated by the appearance of spontaneous synchronized Ca2+ spikes in the neuronal network during reoxygenation after each episode of hypoxia. It is shown that the preconditioning effect occurs only in glutamatergic neurons. In the GABAergic neurons the effect of preconditioning was not observed. The activity of NMDA receptors in these neurons was not suppressed but increased after each episode of hypoxia. At the moment of posthypoxic synchronous Ca2+-spike generation, a global increase of the cytoplasmic Ca2+ concentration occurred in a few of GABAergic neurons, followed by the apoptotic death of these cells. The anti-inflammatory cytokine, interleukin-10 (IL-10) prevented the development of posthypoxic hyperexcitability, inhibiting spontaneous synchronous Ca2+ spike, and protected GABAergic neurons from the death, restoring the preconditioning effect in them. PI3-kinase inhibitors wortmannin and LY294002 prevented the IL-10 protective effect abolishing the inhibiting effect of IL-10 on the generation of the Ca2+ synchronous spike. These findings point out to the leading role of GABAergic neurons in the development of posthypoxic hyperexcitability. We suggest that the reason for posthypoxic hyperexcitability in the network is a weakening of the inhibiting effect of GABAergic neurons. Activation of different signaling pathways leading to activation of PKB- and PKG-dependent phosphorylation in the neurons of this type represents a possible strategy to protect neurons from death during hypoxia. © 2014 Pleiades Publishing, Ltd.

引用

页码：125 / 135

页数：10

共 46 条

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