High frequency plant regeneration via somatic embryogenesis in cell suspension cultures of cowpea, Vigna unguiculata (L.) Walp

被引：22

作者：

Prem Anand R. ^{[1
]}

Ganapathi A. ^{[1
]}

Ramesh Anbazhagan V. ^{[1
]}

Vengadesan G. ^{[1
]}

Selvaraj N. ^{[1
]}

机构：

[1] Department of Biotechnology, School of Life Sciences, Bharathidasan University

来源：

In Vitro Cellular & Developmental Biology - Plant | 2000年 / 36卷 / 6期

关键词：

Abscisic acid; Embryogenic callus; Primary leaves; Shaking speed; Somatic embryo;

D O I：

10.1007/s11627-000-0085-4

中图分类号：

学科分类号：

摘要：

Embryogenic callus was induced from primary leaves of Vigna unguiculata (L.) Walp. in MS medium (Murashige and Skoog, 1962) containing 2,4-dichlorophenoxyacetic acid (2,4-D). Greenish-white, friable embryogenic calluses were used to establish suspension cultures. A shaking speed of 90 rpm and 0.4 ml packed cell volume per 25 ml medium were found to be optimal for maintaining suspension cultures. Globular, heart-shaped and torpedo-shaped embryos were developed in suspension culture containing 4.52 μM 2,4-D. Maturation of cotyledonary-stage somatic embryos was achieved on 0.05 μM 2,4-D, 5 μM abscisic acid and 3% mannitol. Twenty-two percent of the embryos were converted into plants and survived; survival in the field was 8-10%.

引用

页码：475 / 480

页数：5

共 22 条

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