Levels of reduced and oxidized coenzyme Q-10 and 8-hydroxy-2′-deoxyguanosine in the CSF of patients with Alzheimer’s disease demonstrate that mitochondrial oxidative damage and/or oxidative DNA damage contributes to the neurodegenerative process

被引:0
作者
Chiaki Isobe
Takashi Abe
Yasuo Terayama
机构
[1] Iwate Medical University,Department of Neurology
[2] Iwate Medical University,Department of Neurology, Chitose Daiichi Hospital
来源
Journal of Neurology | 2010年 / 257卷
关键词
Alzheimer’s disease; Free radicals; Reduced coenzyme Q-10; Oxidized coenzyme Q-10; Oxidized/total coenzyme Q-10; 8-Hydroxy-2′-deoxyguanosine; Cerebrospinal fluid;
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摘要
To investigate the possibility that mitochondrial oxidative damage, oxidative DNA damage or both contribute to the neurodegenerative process of Alzheimer’s disease (AD), we employed high-performance liquid chromatography using an electrochemical detector to measure the concentrations of the reduced and oxidized forms of coenzyme Q-10 (CoQ-10) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) in the cerebrospinal fluid (CSF) of 30 patients with AD and in 30 age-matched controls with no neurological disease. The percentage of oxidized/total CoQ-10 (%CoQ-10) in the CSF of the AD group (78.2 ± 18.8%) was significantly higher than in the control group (41.3 ± 10.4%) (P < 0.0001). The concentration of 8-OHdG in the CSF of AD patients was greater than in the CSF of controls (P < 0.0001) and was positively correlated with the duration of illness (rs = 0.95, P < 0.0001). The %CoQ-10 was correlated with concentrations of 8-OHdG in the CSF of AD patients (rs = 0.66, P < 0.001). The present study suggests that both mitochondrial oxidative damage and oxidative DNA damage play important roles in the pathogenesis of early AD development.
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页码:399 / 404
页数:5
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