Drug-free macromolecular therapeutics induce apoptosis of patient chronic lymphocytic leukemia cells

被引：0
作者：
Te-Wei Chu
Ken M. Kosak
Paul J. Shami
Jindřich Kopeček
机构：
[1] University of Utah,Department of Pharmaceutics and Pharmaceutical Chemistry
[2] University of Utah,Division of Hematology and Hematologic Malignancies and Huntsman Cancer Institute
[3] University of Utah,Department of Bioengineering
[4] University of Utah,undefined
来源：
Drug Delivery and Translational Research | 2014年 / 4卷
关键词：
CLL; B-cell; CD20; Apoptosis; HPMA copolymer; Nanomedicine;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A new drug-free nanotherapeutic approach for B-cell malignancies was developed. Exposure of B-cells to an anti-CD20 Fab’-morpholino oligonucleotide1 (MORF1) conjugate decorated the cell surface with MORF1; further exposure of the decorated cells to multivalent polymer-oligonucleotide2 conjugates (P-MORF2) resulted in CD20 clustering at the cell surface with induction of apoptosis. We evaluated this concept in chronic lymphocytic leukemia (CLL) cells isolated from ten patients. Apoptosis and cytotoxicity were observed in eight samples, including two samples with the 17p13 deletion, which suggested a p53-independent mechanism of apoptosis induction. When compared to an anti-CD20 monoclonal antibody (mAb), the nanotherapeutic showed significantly more potent apoptosis-inducing activity and cytotoxicity. This was due to the multivalency effect (eight binding sites per polymer chain) of our design in comparison to the divalent mAb. In conclusion, we have developed a novel and potent therapeutic system against CLL and other B-cell malignancies with significant advantages over conventional chemoimmunotherapy.
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页码：389 / 394
页数：5
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