Field trial demonstrating phytoremediation of the military explosive RDX by XplA/XplB-expressing switchgrass

被引：0
作者：
Timothy J. Cary
Elizabeth L. Rylott
Long Zhang
Ryan M. Routsong
Antonio J. Palazzo
Stuart E. Strand
Neil C. Bruce
机构：
[1] US Army Corps of Engineers,Engineer Research and Development Center, Cold Regions Research and Engineering Laboratory, Biogeochemical Sciences Branch
[2] University of York,Centre for Novel Agricultural Products, Department of Biology
[3] University of Washington,Department of Civil and Environmental Engineering
来源：
Nature Biotechnology | 2021年 / 39卷
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摘要：
The explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), a major component of munitions, is used extensively on military training ranges. As a result, widespread RDX pollution in groundwater and aquifers in the United States is now well documented. RDX is toxic, but its removal from training ranges is logistically challenging, lacking cost-effective and sustainable solutions. Previously, we have shown that thale cress (Arabidopsis thaliana) engineered to express two genes, xplA and xplB, encoding RDX-degrading enzymes from the soil bacterium Rhodococcus rhodochrous 11Y can break down this xenobiotic in laboratory studies. Here, we report the results of a 3-year field trial of XplA/XplB-expressing switchgrass (Panicum virgatum) conducted on three locations in a military site. Our data suggest that XplA/XplB switchgrass has in situ efficacy, with potential utility for detoxifying RDX on live-fire training ranges, munitions dumps and minefields.
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页码：1216 / 1219
页数：3
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