Intravital imaging of orthotopic and ectopic bone

被引：0
作者：
Kunihiko Hashimoto
Takashi Kaito
Junichi Kikuta
Masaru Ishii
机构：
[1] Osaka University,Department of Orthopedic Surgery, Graduate School of Medicine
[2] Osaka University,Department of Immunology and Cell Biology, Graduate School of Medicine & Frontier Biosciences
来源：
Inflammation and Regeneration | / 40卷
关键词：
Intravital imaging; Multiphoton microscopy; Regeneration; Bone; Ectopic bone;
D O I：
暂无
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学科分类号：
摘要：
Bone homeostasis is dynamically regulated by a balance between bone resorption by osteoclasts and bone formation by osteoblasts. Visualizing and evaluating the dynamics of bone cells in vivo remain difficult using conventional technologies, including histomorphometry and imaging analysis. Over the past two decades, multiphoton microscopy, which can penetrate thick specimens, has been utilized in the field of biological imaging. Using this innovative technique, the in vivo dynamic motion of bone metabolism-related cells and their interactions has been revealed. In this review, we summarize previous approaches used for bone imaging and provide an overview of current bone tissue imaging methods using multiphoton excitation microscopy.
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