Direct Adventitious Shoot Formation from Apical Shoot Explants of Euphorbia tirucalli

被引:0
作者
Guohua Ma
Jaime A. Teixeira da Silva
Guojiang Wu
机构
[1] The Chinese Academy of Sciences,Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden
[2] Kagawa University,Faculty of Agriculture and Graduate School of Agriculture
来源
Journal of Plant Growth Regulation | 2011年 / 30卷
关键词
Adventitious bud; Root formation; Regeneration;
D O I
暂无
中图分类号
学科分类号
摘要
The induction of adventitious buds from apical shoot explants of Euphorbia tirucalli was studied. On average, 10.5 adventitious buds were efficiently induced in a ring on the segment from one apical explant on MS (Murashige and Skoog) medium supplemented with 0.5 mg l−1 thidiazuron and 0.5 mg l−1 benzylaminopurine. The adventitious buds could develop into adventitious shoots during subsequent cultures on hormone-free MS medium. For rooting, shoot clumps were cultured on half-strength MS medium containing 0.2 mg l−1 α-naphthaleneacetic acid or indole-3-butyric acid. All the rooted plants survived establishment in soil within 2 months.
引用
收藏
页码:114 / 116
页数:2
相关论文
共 87 条
[1]  
Biesboer DD(1979)The effect of medium modification and selected precursors on sterol production by short-term callus cultures of J Nat Prod 42 648-657
[2]  
Mahlberg PG(1980)Hydrocarbons from plants: analytical methods and observations Naturwissenschaften 67 525-533
[3]  
Calvin M(1974)In vitro culture as a method for vegetative propagation of Z Pflanzenphysiol 71 271-274
[4]  
de Langhe E(1975)Steroid hormones in plants Lloydia 38 195-209
[5]  
Debergh P(1989)An antitumor principle from Planta Med 55 271-272
[6]  
Van Rijk R(2004)Expressed sequence tags from callus of Plant Biotechnol 21 349-353
[7]  
Heftmann E(1982): a resource for genes involved in triterpenoid and sterol biosynthesis HortScience 17 533-21
[8]  
Itokawa H(1976)Tissue culture propagation of Science 194 46-497
[9]  
Ichihara Y(1980)The petroleum plant: perhaps we can grow gasoline Hortculture 58 19-137
[10]  
Watanabe K(1962)Petroleum plantations Physiol Plant 15 473-578