Diffusion of Myelin Oligodendrocyte Glycoprotein in Living OLN-93 Cells Investigated by Raster-Scanning Image Correlation Spectroscopy (RICS)

被引：0
作者：
Ellen Gielen
Nick Smisdom
Ben De Clercq
Martin vandeVen
Rik Gijsbers
Zeger Debyser
Jean-Michel Rigo
Johan Hofkens
Yves Engelborghs
Marcel Ameloot
机构：
[1] Catholic University Leuven,Laboratory for Biomolecular Dynamics
[2] Hasselt University and transnationale Universiteit Limburg,Laboratory for Cell Physiology, Biomedical Research Institute
[3] Eindhoven University of Technology,Department of Applied Physics
[4] Catholic University Leuven,Laboratory for Molecular Virology and Gene Therapy
[5] Catholic University Leuven,Laboratory of Photochemistry and Spectroscopy
来源：
Journal of Fluorescence | 2008年 / 18卷
关键词：
Raster-scanning image correlation spectroscopy; Myelin oligodendrocyte glycoprotein; OLN-93; Diffusion; Lipid rafts;
D O I：
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中图分类号：
学科分类号：
摘要：
Many membrane proteins and lipids are partially confined in substructures ranging from tens of nanometers to micrometers in size. Evidence for heterogeneities in the membrane of oligodendrocytes, i.e. the myelin-producing cells of the central nervous system, is almost exclusively based on detergent methods. However, as application of detergents can alter the membrane phase behaviour, it is important to investigate membrane heterogeneities in living cells. Here, we report on the first investigations of the diffusion behavior of the myelin-specific protein MOG (myelin oligodendrocyte glycoprotein) in OLN-93 as studied by the recently developed RICS (raster-scanning image correlation spectroscopy) technique. We implemented RICS on a standard confocal laser-scanning microscope with one-photon excitation and analog detection. Measurements on FITC-dextran were used to evaluate the performance of the system and the data analysis procedure.
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页码：813 / 819
页数：6
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