Isolation and characterization of 21 polymorphic microsatellite loci in the iconic Australian lungfish, Neoceratodus forsteri, using the Ion Torrent next-generation sequencing platform

被引:0
作者
Joel A. Huey
Kathryn M. Real
Peter B. Mather
Vincent Chand
David T. Roberts
Thomas Espinoza
Andrew McDougall
Peter K. Kind
Steven Brooks
Jane M. Hughes
机构
[1] Griffith University,Australian Rivers Institute
[2] Queensland University of Technology,Science and Engineering Faculty
[3] Seqwater,undefined
[4] Queensland Department of Natural Resources and Mines,undefined
[5] Queensland Department of Agriculture,undefined
[6] Fisheries and Forestry,undefined
来源
Conservation Genetics Resources | 2013年 / 5卷
关键词
Lungfish; Ion Torrent; Microsatellite;
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学科分类号
摘要
We isolated and characterized 21 microsatellite loci in the vulnerable and iconic Australian lungfish, Neoceratodus forsteri. Loci were screened across eight individuals from the Burnett River and 40 individuals from the Pine River. Genetic diversity was low with between one and six alleles per locus within populations and a maximum expected heterozygosity of 0.774. These loci will now be available to assess effective population sizes and genetic structure in N. forsteri across its natural range in South East Queensland, Australia.
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页码:737 / 740
页数:3
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