Visualization and identification of hepatitis C viral particles by atomic force microscopy combined with MS/MS analysis

被引：9

作者：

Kaysheva A.L. ^{[1
]}

Ivanov Y.D. ^{[1
]}

Zgoda V.G. ^{[1
]}

Frantsuzov P.A. ^{[1
]}

Pleshakova T.O. ^{[1
]}

Krokhin N.V. ^{[1
]}

Ziborov V.S. ^{[2
]}

Archakov A.I. ^{[1
]}

机构：

[1] Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow 119121

[2] Joint Institute for High Temperatures of Russian Academy of Sciences, Moscow

来源：

Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry | 2010年 / 4卷 / 1期

基金：

俄罗斯基础研究基金会;

关键词：

Atomic force microscopy; Hepatitis C virus HCVcoreAg; Mass spectrometry;

D O I：

10.1134/S1990750810010038

中图分类号：

学科分类号：

摘要：

A possibility of detection and identification of hepatitis C viral (HCV) particles by atomic force microscopy (AFM) in combination with mass spectrometry (MS) has been investigated. The AFM/MS approach is based on two technologies: 1. AFM-biospecific fishing that allows to detect, concentrate from solution and to count protein complexes on the surface of a AFM-nanochip; 2. mass spectrometric identification of these complexes. AFM-biospecific fishing of HCVcoreAg from solution was carried onto the surface of AFM-nanochips with immobilized anti-HCVcoreAg. It was shown that HCVcoreAg/anti-HCVcore im complexes were formed on AFM-nanochips in quantity sufficient for their subsequent mass spectrometric identification. Thus, the AFM/MS approach allows to identify fragments of hepatitis C virus fished on the surface of AFM-nanochip from serum. © 2010 Pleiades Publishing, Ltd.

引用

页码：15 / 24

页数：9

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