In vitro induction of polyploidy in annatto (Bixa orellana)

被引：61

作者：

De Carvalho J.F.R.P. ^{[1
]}

De Carvalho C.R. ^{[1
]}

Otoni W.C. ^{[2
]}

机构：

[1] Departamento de Biologia Geral, Univ. Federal de Viçosa, 36570-000 Viçosa, MG

[2] Departamento de Biologia Vegetal, Univ. Federal de Viçosa, 36570-000 Viçosa, MG

来源：

Plant Cell, Tissue and Organ Culture | 2005年 / 80卷 / 1期

关键词：

Chromosome; Colchicine; Cytogenetic; Oryzalin; Tissue culture;

D O I：

10.1007/s11240-004-8833-5

中图分类号：

学科分类号：

摘要：

The present work aims to establish a protocol for in vitro polyploidization using hypocotyl segments or cotyledonary nodes from in vitro grown annatto seedlings. The culture medium used to induce polyploidization was supplemented with MS salts, B5 vitamin complex, 100 mg l-1 myo-inositol, 3% (w/v) sucrose, 2.28 μM ZEA and 0.30 μM IAA (hypocotyl segments) or 4.56 μM ZEA (cotyledonary nodes), 0.8% (w/v) agar, and different concentrations of microtubule depolymerising agents, namely colchicine (0, 25, 250 and 1250 μM) and oryzalin (0, 5, 15 and 30 μM). To determine the optimum duration of either colchicine or oryzalin treatment for the induction of tetraploids, explants were treated for 15 or 30 days on regeneration medium. High frequencies of polyploidy in regenerated shoots from cotyledonary nodes were achieved in culture medium supplemented with 15 μM oryzalin, for 15 days. Ploidy determination was based on chromosome counting in metaphasic cells from apical buds, and in the number of pairs of heterochromatic markers on the biggest chromosome, as visualized in interphasic nuclei, detection being easier in the latter. Among the characteristics evaluated, the measurements based on stomata length, width, area and frequency enabled greater discrimination between diploid and polyploid regenerated shoots. © Springer 2005.

引用

页码：69 / 75

页数：6

共 38 条

[1]

Adanyia S., Shirai D., In vitro induction of tetraploid ginger (Zinziber officinale Roscoe) and its pollen fertility and germinability, Sci. Hort., 88, pp. 277-287, (2001)

[2]

Borrino E.M., Powell W., Stomatal guard cell length as an indicator of ploidy in microspore-derived plants of barley, Genome, 30, pp. 158-160, (1988)

[3]

Bouvier L., Fillon F.R., Lespinasse Y., Oryzalin as an efficient agent for chromosome doubling of haploid apple shoots in vitro, Plant Breed., 113, pp. 343-346, (1994)

[4]

Carvalho C.R., Saraiva L.S., An air drying technique for maize chromosome without enzymatic maceration, Biotech. Histochem., 68, pp. 142-145, (1993)

[5]

Carvalho C.R., Saraiva L.S., High-resolution HKG-banding in maize mitotic chromosomes, J. Plant Res., 110, pp. 417-420, (1997)

[6]

Chakraborti S.P., Vijayan K., Roy B.N., In vitro induction of tetraploidy in mulberry (Morus alba L.), Plant Cell Rep., 17, pp. 799-803, (1998)

[7]

Compton M.E., Veilleux R.E., Variation for genetic recombination among tomato plants regenerated from three tissue culture systems, Genome, 34, pp. 810-817, (1991)

[8]

Compton M.E., Gray D.J., Elmstrom G.W., Identification of tetraploid regenerants from cotyledons of diploid watermelon cultured in vitro, Euphytica, 87, pp. 165-172, (1996)

[9]

Dolezel J., Lucretti S., Schubert I., Plant chromosome analysis and sorting by flow cytometry, Crit. Rev. Plant Sci., 13, 3, pp. 275-309, (1994)

[10]

Dore C., Evaluation de ploidie des plantes d'une population de choux de Bruxelles (Brassica oleraceae L. spp. gemmifera) d'origine pollinique, Agronomie, 6, pp. 697-701, (1986)

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