Kaempferol Identified by Zebrafish Assay and Fine Fractionations Strategy from Dysosma versipellis Inhibits Angiogenesis through VEGF and FGF Pathways

被引：0
作者：
Fang Liang
Yuxiang Han
Hao Gao
Shengchang Xin
Shaodan Chen
Nan Wang
Wei Qin
Hanbing Zhong
Shuo Lin
Xinsheng Yao
Song Li
机构：
[1] Key Laboratory of Chemical Genomics,Department of Biology
[2] School of Chemical Biology and Biotechnology,Department of Molecular
[3] Peking University Shenzhen Graduate School,undefined
[4] Institute of Traditional Chinese Medicine & Natural Products,undefined
[5] College of Pharmacy,undefined
[6] Jinan University,undefined
[7] South University of Science and Technology of China,undefined
[8] Cell and Developmental Biology,undefined
[9] University of California,undefined
[10] Los Angeles,undefined
来源：
Scientific Reports | / 5卷
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摘要：
Natural products are a rich resource for the discovery of therapeutic substances. By directly using 504 fine fractions from isolated traditional Chinese medicine plants, we performed a transgenic zebrafish based screen for anti-angiogenesis substances. One fraction, DYVE-D3, was found to inhibit the growth of intersegmental vessels in the zebrafish vasculature. Bioassay-guided isolation of DYVE-D3 indicates that the flavonoid kaempferol was the active substance. Kaempferol also inhibited the proliferation and migration of HUVECs in vitro. Furthermore, we found that kaempferol suppressed angiogenesis through inhibiting VEGFR2 expression, which can be enhanced by FGF inhibition. In summary, this study shows that the construction of fine fraction libraries allows efficient identification of active substances from natural products.
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