Stripe rust resistance and genes in Chinese wheat cultivars and breeding lines

被引：1
作者：
Qing-Dong Zeng
De-Jun Han
Qi-Lin Wang
Feng-Ping Yuan
Jian-Hui Wu
Li Zhang
Xiao-Jie Wang
Li-Li Huang
Xian-Ming Chen
Zhen-Sheng Kang
机构：
[1] Northwest A&F University,State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant Protection
[2] Northwest A&F University,State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy
[3] Washington State University,Wheat Genetics, Quality, Physiology, and Disease Research Unit, Agricultural Research Service, United States Department of Agriculture
[4] Washington State University,Department of Plant Pathology
来源：
Euphytica | 2014年 / 196卷
关键词：
Adult plant resistance; Molecular marker; f. sp; genes;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important diseases on wheat in China. To assess resistance in wheat cultivars and breeding lines in China, 330 leading cultivars and 164 advanced breeding lines were evaluated with stripe rust. In the greenhouse tests, seedlings of the entries were inoculated separately with several Pst pathotypes. In the field tests, the entries were evaluated for stripe rust resistance in Yangling, Shaanxi Province artificially inoculated and in Tianshui, Gansu Province under natural infection of Pst. The oversummering/wintering and spring epidemic zones of resistance genes were postulated using molecular markers for Yr5, Yr9, Yr10, Yr15, Yr17, Yr18, and Yr26, in combination with resistance spectra. Out of the 494 wheat entries, 16 (3.24 %) entries had all-stage resistance (ASR) in all race tests, 99 (20.04 %) had adult-plant resistance (APR), 28 (5.67 %) were considered to have slow-rusting (SR), and 351 (71.05 %) were susceptible to one or more races in both seedling and adult-plant stages. Advanced breeding lines had a higher percentage (37.2 %) of resistant entries (The sum of ASR, APR and SR) than leading cultivars (24.85 %). Among the epidemic regions, southern Gansu had a higher percentage of resistant entries than any other regions. Based on stripe rust reactions and molecular markers, two cultivars were found to possibly have Yr5 while no entries have Yr10 or Yr15. Resistance genes Yr9, Yr17, Yr18, and Yr26 were found in 134 (29.4 %), 45 (9.1 %), 10 (2 %), and 15 (3 %) entries, respectively.
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页码：271 / 284
页数：13
相关论文
共 171 条
[1]  
An Z(2009)A silver staining procedure for nucleic acids in polyacrylamide gels without fixation and pretreatment Analyt Biochem 391 77-79
[2]  
Xie L(2005)Epidemiology and control of stripe rust [ Can J Plant Pathol 27 314-317
[3]  
Cheng H(2003) f. sp. Crop Sci 43 2058-2064
[4]  
Zhou Y(2009)] on wheat Plant Dis 93 1093-1101
[5]  
Zhang Q(1995)Development of sequence tagged site and cleaved amplified polymorphic sequence markers for wheat stripe rust resistance gene Theor Appl Genet 90 636-642
[6]  
He X(2003)Race dynamics, diversity, and virulence evolution in Crop Sci 43 1839-1847
[7]  
Huang H(2006) f. sp. Theor Appl Genet 114 21-30
[8]  
Chen XM(2009), the causal agent of wheat stripe rust in China from 2003 to 2007 Theor Appl Genet 119 889-898
[9]  
Chen XM(2006)Conversion of a RAPD-generated PCR product, containing a novel dispersed repetitive element, into a fast and robust assay for the presence of rye chromatin in wheat Theor Appl Genet 112 1434-1440
[10]  
Soria MA(2007)PCR assays for the Theor Appl Genet 114 1277-1287
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