Chitin-fibroin-hydroxyapatite membrane for guided bone regeneration: micro-computed tomography evaluation in a rat model

被引：10

作者：

Baek Y.-J. ^{[1
]}

Kim J.-H. ^{[1
]}

Song J.-M. ^{[1
]}

Yoon S.-Y. ^{[2
]}

Kim H.-S. ^{[3
]}

Shin S.-H. ^{[1
]}

机构：

[1] Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Mulgeum-eup, Yangsan

[2] Department of Oral and Maxillofacial Surgery, Good Gang-An Hospital, Busan

[3] Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University, Miryang

来源：

Maxillofacial Plastic and Reconstructive Surgery | / 38卷 / 1期

关键词：

Chitin-fibroin-hydroxyapatite; Guided bone regeneration; Micro-computed tomography; Rat calvarial defect;

D O I：

10.1186/s40902-016-0060-6

中图分类号：

学科分类号：

摘要：

Background: In guided bone regeneration (GBR) technique, many materials have been used for improving biological effectiveness by adding on membranes. The new membrane which was constructed with chitin-fibroin-hydroxyapatite (CNF/HAP) was compared with a collagen membrane (Bio-Gide®) by means of micro-computed tomography. Methods: Fifty-four rats were used in this study. A critical-sized (8 mm) bony defect was created in the calvaria with a trephine bur. The CNF/HAP membrane was prepared by thermally induced phase separation. In the experimental group (n = 18), the CNF/HAP membrane was used to cover the bony defect, and in the control group (n = 18), a resorbable collagen membrane (Bio-Gide®) was used. In the negative control group (n = 18), no membrane was used. In each group, six animals were euthanized at 2, 4, and 8 weeks after surgery. The specimens were analyzed using micro-CT. Results: Bone volume (BV) and bone mineral density (BMD) of the new bone showed significant difference between the negative control group and membrane groups (P < 0.05). However, between two membranes, the difference was not significant. Conclusions: The CNF/HAP membrane has significant effect on the new bone formation and has the potential to be applied for guided bone regeneration. © 2016, Baek et al.

引用

共 36 条

[31]

Chandy T., Sharma C.P., Chitosan-as a biomaterial, Biomaterials, artificial cells and artificial organs, 18, 1, pp. 1-24, (1990)

[32]

VandeVord P.J., Matthew H.W., DeSilva S.P., Mayton L., Wu B., Wooley P.H., Evaluation of the biocompatibility of a chitosan scaffold in mice, J Biomed Mater Res, 59, 3, pp. 585-590, (2002)

[33]

Onishi H., Machida Y., Biodegradation and distribution of water-soluble chitosan in mice, Biomaterials, 20, 2, pp. 175-182, (1999)

[34]

Jansen J.A., de Ruijter J.E., Janssen P.T., Paquay Y.G., Histological evaluation of a biodegradable polyactive®/hydroxyapatite membrane, Biomaterials, 16, 11, pp. 819-827, (1995)

[35]

Buser D., Dahlin C., Schenk R.K., Guided bone regeneration in implant dentistry, (1994)

[36]

Schmid J., Hammerle C.H., Fliickiger L., Winkler J.R., Olah A.J., Gogolewskiz S., Lang N.P., Blood‐filled spaces with and without filler materials in guided bone regeneration. A comparative experimental study in the rabbit using bioresorbable membranes, Clinical Oral Implants Research, 8, 2, pp. 75-81, (1997)

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