Chitin-fibroin-hydroxyapatite membrane for guided bone regeneration: micro-computed tomography evaluation in a rat model

被引：10
作者：
Baek Y.-J. [1 ]
Kim J.-H. [1 ]
Song J.-M. [1 ]
Yoon S.-Y. [2 ]
Kim H.-S. [3 ]
Shin S.-H. [1 ]
机构：
[1] Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Mulgeum-eup, Yangsan
[2] Department of Oral and Maxillofacial Surgery, Good Gang-An Hospital, Busan
[3] Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University, Miryang
来源：
Maxillofacial Plastic and Reconstructive Surgery | / 38卷 / 1期
关键词：
Chitin-fibroin-hydroxyapatite; Guided bone regeneration; Micro-computed tomography; Rat calvarial defect;
D O I：
10.1186/s40902-016-0060-6
中图分类号：
学科分类号：
摘要：
Background: In guided bone regeneration (GBR) technique, many materials have been used for improving biological effectiveness by adding on membranes. The new membrane which was constructed with chitin-fibroin-hydroxyapatite (CNF/HAP) was compared with a collagen membrane (Bio-Gide®) by means of micro-computed tomography. Methods: Fifty-four rats were used in this study. A critical-sized (8 mm) bony defect was created in the calvaria with a trephine bur. The CNF/HAP membrane was prepared by thermally induced phase separation. In the experimental group (n = 18), the CNF/HAP membrane was used to cover the bony defect, and in the control group (n = 18), a resorbable collagen membrane (Bio-Gide®) was used. In the negative control group (n = 18), no membrane was used. In each group, six animals were euthanized at 2, 4, and 8 weeks after surgery. The specimens were analyzed using micro-CT. Results: Bone volume (BV) and bone mineral density (BMD) of the new bone showed significant difference between the negative control group and membrane groups (P < 0.05). However, between two membranes, the difference was not significant. Conclusions: The CNF/HAP membrane has significant effect on the new bone formation and has the potential to be applied for guided bone regeneration. © 2016, Baek et al.
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