Chitin-fibroin-hydroxyapatite membrane for guided bone regeneration: micro-computed tomography evaluation in a rat model

被引：10
作者：
Baek Y.-J. [1 ]
Kim J.-H. [1 ]
Song J.-M. [1 ]
Yoon S.-Y. [2 ]
Kim H.-S. [3 ]
Shin S.-H. [1 ]
机构：
[1] Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Mulgeum-eup, Yangsan
[2] Department of Oral and Maxillofacial Surgery, Good Gang-An Hospital, Busan
[3] Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University, Miryang
来源：
Maxillofacial Plastic and Reconstructive Surgery | / 38卷 / 1期
关键词：
Chitin-fibroin-hydroxyapatite; Guided bone regeneration; Micro-computed tomography; Rat calvarial defect;
D O I：
10.1186/s40902-016-0060-6
中图分类号：
学科分类号：
摘要：
Background: In guided bone regeneration (GBR) technique, many materials have been used for improving biological effectiveness by adding on membranes. The new membrane which was constructed with chitin-fibroin-hydroxyapatite (CNF/HAP) was compared with a collagen membrane (Bio-Gide®) by means of micro-computed tomography. Methods: Fifty-four rats were used in this study. A critical-sized (8 mm) bony defect was created in the calvaria with a trephine bur. The CNF/HAP membrane was prepared by thermally induced phase separation. In the experimental group (n = 18), the CNF/HAP membrane was used to cover the bony defect, and in the control group (n = 18), a resorbable collagen membrane (Bio-Gide®) was used. In the negative control group (n = 18), no membrane was used. In each group, six animals were euthanized at 2, 4, and 8 weeks after surgery. The specimens were analyzed using micro-CT. Results: Bone volume (BV) and bone mineral density (BMD) of the new bone showed significant difference between the negative control group and membrane groups (P < 0.05). However, between two membranes, the difference was not significant. Conclusions: The CNF/HAP membrane has significant effect on the new bone formation and has the potential to be applied for guided bone regeneration. © 2016, Baek et al.
引用
收藏
相关论文
共 36 条
[1]  
Ueyama Y., Ishikawa K., Mano T., Koyama T., Nagatsuka H., Suzuki K., Et al., Usefulness as guided bone regeneration membrane of the alginate membrane, Biomaterials, 23, 9, pp. 2027-2033, (2002)
[2]  
Carvalho R.S., Nelson D., Kelderman H., Wise R., Guided bone regeneration to repair an osseous defect, Am J Orthod Dentofacial Orthop, 123, 4, pp. 455-467, (2003)
[3]  
Boyne P.J., Regeneration of alveolar bone beneath cellulose acetate filter implants, J Dental Res, 43, 5, (1964)
[4]  
Dupoirieux L., Pourquier D., Picot M.C., Neves M., Comparative study of three different membranes for guided bone regeneration of rat cranial defects, Int J Oral Maxillofac Surg, 30, 1, pp. 58-62, (2001)
[5]  
Teng S.H., Lee E.J., Wang P., Shin D.S., Kim H.E., Three‐layered membranes of collagen/hydroxyapatite and chitosan for guided bone regeneration, J Biomed Mater Res B Appl Biomater, 87, 1, pp. 132-138, (2008)
[6]  
Taguchi Y., Amizuka N., Nakadate M., Ohnishi H., Fujii N., Oda K., Et al., A histological evaluation for guided bone regeneration induced by a collagenous membrane, Biomaterials, 26, 31, pp. 6158-6166, (2005)
[7]  
Song J.M., Shin S.H., Kim Y.D., Lee J.Y., Baek Y.J., Yoon S.Y., Et al., Comparative study of chitosan/fibroin-hydroxyapatite and collagen membranes for guided bone regeneration in rat calvarial defects: Micro-computed tomography analysis, Int J Oral Sci. Nature Publishing Group, 6, 2, pp. 87-93, (2014)
[8]  
Shi C., Zhu Y., Ran X., Wang M., Su Y., Cheng T., Therapeutic Potential of Chitosan and Its Derivatives in Regenerative Medicine, J Surg Res, 133, 2, pp. 185-192, (2006)
[9]  
Jin X., Stephen P Mccarthy A.A., Grosskaplan D.L., Chitosan Film Acylation and Effects on Biodegradability, Macromolecules., 29, 10, pp. 3436-3440, (1996)
[10]  
Zhang R., Ma P.X., Poly(Α-hydroxyl acids)/hydroxyapatite porous composites for bone-tissue engineering. I. Preparation and morphology, J Biomed Mater Res, 44, 4, pp. 446-455, (1999)
1234