Comparison of enzymatic activities of lipases from Burkholderia plantarii and Burkholderia cepacia

被引:0
|
作者
Saori Kataoka
Sayuri Kawamoto
Kazunobu Tsumura
Kazuhiko Ishikawa
机构
[1] Fuji Oil Holdings Inc.,Research Institute for Creating the Future
[2] National Institute of Advanced Industrial Science and Technology (AIST),Biomedical Research Institute
来源
Archives of Microbiology | 2023年 / 205卷
关键词
Lipase; Interesterification; Hydrolysis;
D O I
暂无
中图分类号
学科分类号
摘要
Lipases (EC 3.1.1.3) are enzymes used in the oils and fats industries to modify the physicochemical properties of triacylglycerol (TAG). Lipase-catalyzed interesterification at high temperatures is an effective method for modifying the physicochemical properties of TAG. The lipase from Burkholderia plantarii (BpL) exhibits excellent catalytic activity for non-regiospecific interesterification at high temperatures, with depressed lipase hydrolytic activity. The detailed catalytic mechanism for reactions involving catalytic residues has not been elucidated because of the lack of a conventional method for estimating interesterification activity. We used our original water-in-oil emulsion system to estimate the interesterification activity of lipases. BpL showed 10% hydrolytic and 140% interesterification activities compared to the lipase from Burkholderia cepacia, which has a high sequence homology with BpL. By comparing the sequence and crystal structure data of the lipases, we clarified that two amino acids near the active center are one of the factors controlling the hydrolytic and interesterification activities of the enzyme.
引用
收藏
相关论文
共 50 条
  • [1] Comparison of enzymatic activities of lipases from Burkholderia plantarii and Burkholderia cepacia
    Kataoka, Saori
    Kawamoto, Sayuri
    Tsumura, Kazunobu
    Ishikawa, Kazuhiko
    ARCHIVES OF MICROBIOLOGY, 2023, 205 (09)
  • [2] Structural and functional insights into the enzymatic activities of lipases from Burkholderia stagnalis and Burkholderia plantarii
    Kataoka, Saori
    Kawamoto, Sayuri
    Kitagawa, Sayuri
    Kugimiya, Wataru
    Tsumura, Kazunobu
    Akutsu, Yukie
    Kubota, Tomomi
    Ishikawa, Kazuhiko
    FEBS LETTERS, 2024, 598 (11) : 1411 - 1421
  • [3] Synthetically useful variants of industrial lipases from Burkholderia cepacia and Pseudomonas fluorescens
    Yoshida, Kazunori
    Ono, Masakazu
    Yamamoto, Takahiro
    Utsumi, Takashi
    Koikeda, Satoshi
    Ema, Tadashi
    ORGANIC & BIOMOLECULAR CHEMISTRY, 2017, 15 (41) : 8713 - 8719
  • [4] Development and application of an efficient recombineering system for Burkholderia glumae and Burkholderia plantarii
    Li, Ruijuan
    Shi, Hongbo
    Zhao, Xiaoyu
    Liu, Xianqi
    Duan, Qiong
    Song, Chaoyi
    Chen, Hanna
    Zheng, Wentao
    Shen, Qiyao
    Wang, Maoqin
    Wang, Xue
    Gong, Kai
    Yin, Jia
    Zhang, Youming
    Li, Aiying
    Fu, Jun
    MICROBIAL BIOTECHNOLOGY, 2021, 14 (04): : 1809 - 1826
  • [5] Burkholderia cepacia from a sink drain
    Moore, JE
    Thompson, I
    Crowe, M
    Xu, J
    Shaw, A
    Millar, BC
    Redmond, AOB
    Reid, AJM
    Clarke, C
    Elborn, JS
    JOURNAL OF HOSPITAL INFECTION, 2002, 50 (03) : 235 - 237
  • [6] Burkholderia cepacia infections
    Loukil, C
    Bidet, P
    Vandamme, P
    Bingen, E
    MEDECINE ET MALADIES INFECTIEUSES, 2002, 32 : 137S - 138S
  • [7] DNA-based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III
    Mahenthiralingam, E
    Bischof, J
    Byrne, SK
    Radomski, C
    Davies, JE
    Av-Gay, Y
    Vandamme, P
    JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (09) : 3165 - 3173
  • [8] Discrimination of Burkholderia multivorans and Burkholderia vietnamiensis from Burkholderia cepacia genomovars I, III, and IV by PCR
    Bauernfeind, A
    Schneider, I
    Jungwirth, R
    Roller, C
    JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (05) : 1335 - 1339
  • [9] Antibacterial iminopyrrolidines from Burkholderia plantarii, a bacterial pathogen of rice
    Mitchell, RE
    Teh, KL
    ORGANIC & BIOMOLECULAR CHEMISTRY, 2005, 3 (19) : 3540 - 3543
  • [10] Enzymatic synthesis of carnosine derivatives catalysed by Burkholderia cepacia lipase
    D'Arrigo, Paola
    Kanerva, Liisa T.
    Li, Xiang-Guo
    Saraceno, Caterina
    Servi, Stefano
    Tessaro, Davide
    TETRAHEDRON-ASYMMETRY, 2009, 20 (14) : 1641 - 1645