Comparison of enzymatic activities of lipases from Burkholderia plantarii and Burkholderia cepacia
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作者:
Saori Kataoka
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机构:Fuji Oil Holdings Inc.,Research Institute for Creating the Future
Saori Kataoka
Sayuri Kawamoto
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机构:Fuji Oil Holdings Inc.,Research Institute for Creating the Future
Sayuri Kawamoto
Kazunobu Tsumura
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机构:Fuji Oil Holdings Inc.,Research Institute for Creating the Future
Kazunobu Tsumura
Kazuhiko Ishikawa
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机构:Fuji Oil Holdings Inc.,Research Institute for Creating the Future
Kazuhiko Ishikawa
机构:
[1] Fuji Oil Holdings Inc.,Research Institute for Creating the Future
[2] National Institute of Advanced Industrial Science and Technology (AIST),Biomedical Research Institute
来源:
Archives of Microbiology
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2023年
/
205卷
关键词:
Lipase;
Interesterification;
Hydrolysis;
D O I:
暂无
中图分类号:
学科分类号:
摘要:
Lipases (EC 3.1.1.3) are enzymes used in the oils and fats industries to modify the physicochemical properties of triacylglycerol (TAG). Lipase-catalyzed interesterification at high temperatures is an effective method for modifying the physicochemical properties of TAG. The lipase from Burkholderia plantarii (BpL) exhibits excellent catalytic activity for non-regiospecific interesterification at high temperatures, with depressed lipase hydrolytic activity. The detailed catalytic mechanism for reactions involving catalytic residues has not been elucidated because of the lack of a conventional method for estimating interesterification activity. We used our original water-in-oil emulsion system to estimate the interesterification activity of lipases. BpL showed 10% hydrolytic and 140% interesterification activities compared to the lipase from Burkholderia cepacia, which has a high sequence homology with BpL. By comparing the sequence and crystal structure data of the lipases, we clarified that two amino acids near the active center are one of the factors controlling the hydrolytic and interesterification activities of the enzyme.
机构:
Fuji Oil Holdings Inc, Res Inst Creating Future, Tsukubamirai, JapanFuji Oil Holdings Inc, Res Inst Creating Future, Tsukubamirai, Japan
Kataoka, Saori
Kawamoto, Sayuri
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机构:
Fuji Oil Holdings Inc, Res Inst Creating Future, Tsukubamirai, JapanFuji Oil Holdings Inc, Res Inst Creating Future, Tsukubamirai, Japan
Kawamoto, Sayuri
Kitagawa, Sayuri
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机构:
Fuji Oil Holdings Inc, Res Inst Creating Future, Tsukubamirai, JapanFuji Oil Holdings Inc, Res Inst Creating Future, Tsukubamirai, Japan
Kitagawa, Sayuri
Kugimiya, Wataru
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机构:
Fuji Oil Holdings Inc, Res Inst Creating Future, Tsukubamirai, JapanFuji Oil Holdings Inc, Res Inst Creating Future, Tsukubamirai, Japan
Kugimiya, Wataru
Tsumura, Kazunobu
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机构:
Fuji Oil Holdings Inc, Res Inst Creating Future, Tsukubamirai, JapanFuji Oil Holdings Inc, Res Inst Creating Future, Tsukubamirai, Japan
Tsumura, Kazunobu
Akutsu, Yukie
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机构:
Natl Inst Adv Ind Sci & Technol, Biomed Res Inst, Tsukuba, JapanFuji Oil Holdings Inc, Res Inst Creating Future, Tsukubamirai, Japan
Akutsu, Yukie
Kubota, Tomomi
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机构:
Natl Inst Adv Ind Sci & Technol, Biomed Res Inst, Tsukuba, JapanFuji Oil Holdings Inc, Res Inst Creating Future, Tsukubamirai, Japan
Kubota, Tomomi
Ishikawa, Kazuhiko
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机构:
Natl Inst Adv Ind Sci & Technol, Biomed Res Inst, Tsukuba, Japan
Matsutani Chem Ind Co Ltd, 5-3 Kita Itami, Itami, Hyogo 6648508, JapanFuji Oil Holdings Inc, Res Inst Creating Future, Tsukubamirai, Japan