Study of kinetics of thermal aggregation of mitochondrial aspartate aminotransferase by dynamic light scattering: protective effect of α-crystallin

被引：0
作者：
Nikolay V. Golub
Kira A. Markossian
Mikhail V. Sholukh
Konstantin O. Muranov
Boris I. Kurganov
机构：
[1] Russian Academy of Sciences,Bach Institute of Biochemistry
[2] Belarusian State University,Emanuel Institute of Biochemical Physics
[3] Russian Academy of Sciences,undefined
来源：
European Biophysics Journal | 2009年 / 38卷
关键词：
Mitochondrial aspartate aminotransferase; Aggregation; α-Crystallin; Dynamic light scattering;
D O I：
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学科分类号：
摘要：
Thermal aggregation of aspartate aminotransferase from pig heart mitochondria (mAAT) has been studied at various temperatures and various protein concentrations by dynamic light scattering. The character of the dependence of protein aggregate size on time indicates that aggregation of mAAT proceeds in the regime of diffusion-limited cluster–cluster aggregation. Suppression of mAAT aggregation by α-crystallin is due to transition of the aggregation process into the regime of reaction-limited cluster–cluster aggregation. Realization of this regime of aggregation means that the sticking probability for the colliding particles is less than unity.
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页码：547 / 556
页数：9
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