Binding interactions of pefloxacin mesylate with bovine lactoferrin and human serum albumin.

被引：29

作者：

Fan J.C. ^{[1
]}

Chen X. ^{[1
]}

Wang Y. ^{[1
]}

Fan C.P. ^{[1
]}

Shang Z.C. ^{[1
]}

机构：

[1] Department of Chemistry, Zhejiang University, Hangzhou

来源：

Journal of Zhejiang University SCIENCE B | 2006年 / 7卷 / 6期

基金：

中国国家自然科学基金;

关键词：

Pefloxacin mesylate (PFLX); Bovine lactoferrin (BLf); Human serum albumin (HSA); Fluorescence spectra; Energy-transfer efficiency; O657.3;

D O I：

10.1631/jzus.2006.B0452

中图分类号：

学科分类号：

摘要：

The binding of pefloxacin mesylate (PFLX) to bovine lactoferrin (BLf) and human serum albumin (HSA) in dilute aqueous solution was studied using fluorescence spectra and absorbance spectra. The binding constant K and the binding sites n were obtained by fluorescence quenching method. The binding distance r and energy-transfer efficiency E between pefloxacin mesylate and bovine lactoferrin as well as human serum albumin were also obtained according to the mechanism of Förster-type dipole-dipole nonradiative energy-transfer. The effects of pefloxacin mesylate on the conformations of bovine lactoferrin and human serum albumin were also analyzed using synchronous fluorescence spectroscopy.

引用

页码：452 / 458

页数：6

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