Time-Dependent Oxidative Stress Responses of Crucian Carp (Carassius auratus) to Intraperitoneal Injection of Extracted Microcystins

被引：0
作者：
X. Zhang
P. Xie
D. Li
R. Tang
H. Lei
Y. Zhao
机构：
[1] Fisheries College of Huazhong Agricultural University,Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology
[2] Chinese Academy of Sciences,undefined
来源：
Bulletin of Environmental Contamination and Toxicology | 2009年 / 82卷
关键词：
Crucian carp; Extracted microcystins; Oxidative stress; Time-dependent;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
This study was conducted to investigate time-dependent changes in oxidative enzymes in liver of crucian carp after intraperitoneally injection with extracted microcystins 600 and 150 μg kg−1 body weight. The results showed that activities of antioxidant enzymes, including superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase generally exhibited a rapid increase in early phase (1–3 h post injection), but gradually decreased afterwards (12–48 h) compared with the control, with an evident time-dependent effect. These zigzag changes over time contributed a better understanding on oxidative stress caused by microcystins in fish.
引用
收藏
页码：574 / 578
页数：4
相关论文
共 58 条
[1]  
Beers RF(1952)A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase J Biol Chem 195 133-140
[2]  
Sizer IW(1976)A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding Anal Biochem 72 248-254
[3]  
Bradford M(1975)Purification and characterization of the flavoenzyme glutathione reductase from rat liver J Biol Chem 250 5475-5480
[4]  
Carlberg I(1992)Cytoskeletal changes in hepatocytes induced by microcystis toxins and their relation to hyperphosphorylation of cell proteins Chem Biol Interact 81 181-196
[5]  
Mannervik B(2002)An international intercomparison exercise for the determination of purified microcystin-LR and microcystins in cyanobacterial field material Anal Biochem Chem 374 437-444
[6]  
Falconer IR(2003)Chronic cold exposure affects the antioxidant defense system in various rat tissues Clin Chim Acta 333 69-77
[7]  
Yeung DSK(1976)Glutathione peroxidase activity in selenium deficient rat liver Biochem Biophys Res Commun 71 952-958
[8]  
Fastner J(2003)Responses of antioxidant systems in the hepatocytes of common carp ( Toxicon 42 85-89
[9]  
Codd GA(1969) L.) to the toxicity of microcystin-LR J Biol Chem 244 6049-6055
[10]  
Metcalf JS(2005)Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein) Toxicon 45 395-402
123456