Kinetic Analysis of the ex vivo Expansion of Human Hematopoietic Stem/Progenitor Cells

被引：0
作者：
Raquel Gonçalves
Cláudia Lobato da Silva
Bruno S. Ferreira
Maria Amélia N. D. A. Lemos
Francisco Lemos
Graça Almeida-Porada
Joaquim M. S. Cabral
机构：
[1] Instituto Superior Técnico,Centro de Engenharia Biológica e Química
[2] University of Nevada,Department of Animal Biotechnology
来源：
Biotechnology Letters | 2006年 / 28卷
关键词：
cell expansion; expansion kinetics model; human hematopoietic cells; human stem cells; stroma;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The total cell expansion of human umbilical cord blood (CB) and adult bone marrow (BM) CD34+-enriched cells cultured in supplemented serum-free media, either over irradiated human feeder layers or in stroma-free systems, were characterized by a simple kinetic model using only two parameters: the specific cell expansion rate, μ, and the death rate constant, kk. Both CB and BM cells can expand at approximately the same rate (0.21 day−1) in this culture system however, cell death depends on the presence of stroma and the environment in which the cells are cultured.
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页码：335 / 340
页数：5
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