Effects of the sporulation conditions on the lovastatin production by Aspergillus terreus

被引：0
作者：
E. Rodríguez Porcel
J. L. Casas López
M. A. Vilches Ferrón
J. A. Sánchez Pérez
J. L. García Sánchez
Y. Chisti
机构：
[1] University of Almería,Department of Chemical Engineering
[2] Massey University,Institute of Technology and Engineering
来源：
Bioprocess and Biosystems Engineering | 2006年 / 29卷
关键词：
Lovastatin; Fermentation; Sporulation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The production of biomass and lovastatin by spore-initiated submerged fermentations of Aspergillus terreus ATCC 20542 was shown to depend on the age of the spores used for inoculation. Cultures started from older spores produced significantly higher titers of lovastatin. For example, the lovastatin titer increased by 52% when the spore age at inoculation rose from 9 to 16 days. The lovastatin titer for a spore age of 16 days was 186.5±20.1 mg L−1. The time to sporulation on surface cultures was sensitive to the light exposure history of the fungus and the spore inoculation concentration levels. A light exposure level of 140 μE m−2 s−1 and a spore concentration of 1,320 spore cm−2 produced the greatest extent of sporulation within about 50 h of inoculation. Sporulation was slowed in the dark and with diluted inoculants. A rigorous analysis of the data of statistically designed experiments showed the above observations to be highly reproducible.
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页码：1 / 5
页数：4
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共 69 条
[1]  
Alberts AW(1980)Mevinolin: a highly potent competitive inhibitor of hydroxymethylglutaryl-coenzyme A reductase and cholesterol-lowering agent Proc Natl Acad Sci USA 77 3957-3961
[2]  
Chen J(2003)Production of lovastatin by Enzyme Microb Technol 33 270-277
[3]  
Kuron G(1976): effects of the C:N ratio and the principal nutrients on growth and metabolite production J Antibiot 29 1346-1348
[4]  
Hunt V(1976)ML-236 A, ML236 B and ML-236 C, new inhibitors of cholesterogenesis produced by FEBS Lett 72 323-326
[5]  
Huff J(2005)Competitive inhibition of 3-hydroxy-3-methyl glutaryl coenzyme A reductase by ML-236 A and ML236 B fungal metabolites having hypocholesterolemic activity Fungal Genet Biol 42 471-481
[6]  
Hoffman C(1995)Identification and expression analysis of regulatory genes induced during conidiation in J Chromatogr A 704 363-367
[7]  
Rothrock J(1992)High-performance liquid chromatographic analysis of mevinolin as mevinolinic acid in fermentation broths Bioprocess Eng 8 1-7
[8]  
Lopez M(1996)Interactions of cell morphology and transport processes in the lovastatin fermentation J Ind Microbiol 16 163-170
[9]  
Joshua H(2000)Secondary metabolites of the fungus Process Biochem 36 363-368
[10]  
Harris E(1998): a review Biotechnol Tech 12 529-532
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