Diversity in the disulfide folding pathways of cystine knot peptides

被引:0
|
作者
Norelle L. daly
Richard J. clark
Ulf Göransson
David J. craik
机构
[1] University of Queensland,Institute for Molecular Bioscience
来源
Letters in Peptide Science | 2003年 / 10卷
关键词
circular proteins; cyclotides; kalata B1; protein folding;
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学科分类号
摘要
The plant cyclotides are a fascinating family of circular proteins that contain a cyclic cystine knot motif (CCK). This unique family was discovered only recently but contains over 50 known sequences to date. Various biological activities are associated with these peptides including antimicrobial and insecticidal activity. The knotted topology and cyclic nature of the cyclotides poses interesting questions about the folding mechanisms and how the knotted arrangement of disulfide bonds is formed. Some studies have been performed on related inhibitor cystine knot (ICK) containing peptides, but little is known about the folding mechanisms of CCK molecules. We have examined the oxidative refolding and reductive unfolding of the prototypic member of the cyclotide family, kalata B1. Analysis of the rates of formation of the intermediates along the reductive unfolding pathway highlights the stability conferred by the cystine knot motif. Significant differences are observed between the folding of kalata B1 and an acyclic cystine knot protein, EETI-II, suggesting that the circular backbone has a significant influence in directing the folding pathway.
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页码:523 / 531
页数:8
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