Cytotoxic and apoptotic effects of Ferula gummosa Boiss: extract on human breast adenocarcinoma cell line

被引:0
作者
Rashidi, Roghayeh [1 ]
Roohbakhsh, Ali [2 ]
Mohtashami, Leila [3 ]
Mobasheri, Leila [1 ]
Kheradmand, Hamidreza [4 ]
Amiri, Mohammad Sadegh [5 ]
Ghorbani, Ahmad [1 ]
Mousavi, Seyed Hadi [4 ,5 ]
机构
[1] Mashhad Univ Med Sci, Pharmacol Res Ctr Med Plants, Mashhad, Iran
[2] Mashhad Univ Med Sci, Pharmaceut Technol Inst, Pharmaceut Res Ctr, Mashhad, Iran
[3] Mashhad Univ Med Sci, Sch Pharm, Dept Pharmacognosy, Mashhad, Iran
[4] Mashhad Univ Med Sci, Med Toxicol Res Ctr, Sch Med, Mashhad, Iran
[5] Payame Noor Univ, Dept Biol, Tehran, Iran
关键词
Ferula gummosa Boiss; Breast cancer; Apoptosis; Oxidative stress; Metastasis; VINCRISTINE CYTOTOXICITY; CARCINOMA CELLS; LEAF EXTRACTS; ESSENTIAL OIL; GENUS-FERULA; GUM; UMBELLIPRENIN; ANTIOXIDANT; COMBINATION; INDUCTION;
D O I
10.1007/s11033-024-09364-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Purpose Ferula gummosa Boiss. is a well-known and valuable medicinal plant in Iran. Research has shown that this plant has several pharmacological properties, including anti-bacterial, anti-cancer and etc. In the present study, we investigated the cytotoxic properties of F. gummosa Boiss. extract in MCF-7 breast adenocarcinoma cells. Methods The cytotoxicity and pro-apoptotic properties of the extract were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test and propidium iodide (PI) stained cells, respectively. Apoptosis and necrosis were evaluated by annexin V-PI staining. The levels of reactive oxygen species (ROS),malondialdehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD) was determined to evaluate oxidative stress. The cell migration and the gene expression were assessed by scratch assay and quantitative real-time polymerase chain reaction (q-RT-PCR), respectively. Results The extract of F. gummosa decreased the viability and cell cycle progression of MCF-7 cells by inducing apoptosis and necrosis, increasing ROS and MDA levels, and decreasing GSH levels and SOD activity. It also lowered the cells' migration capability by enhancing p53 mRNA levels and reducing MMP-9 mRNA expression. Conclusion F. gummosa exhibited pro-apoptotic, anti-proliferative, and anti-metastatic effects on MCF-7 cells. It is therefore recommended that detailed future research be done on different parts of the plant or its secondary metabolites to find anti-cancer lead compounds.
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页数:11
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