Generation of New Benanomicin Analogues by Biotransformation Using Escherichia coli Expressing Actinomycete Cytochrome P450

被引：0
作者：
Hiroyuki Kumagai
Maya Umekita
Ryuichi Sawa
Yoshikazu Takahashi
Akira Arisawa
Kunio Isshiki
Yoshio Nishimura
Yuzuru Akamatsu
机构：
[1] Microbial Chemistry Research Center,
[2] Bioresource Laboratories,undefined
[3] Mercian Corp.,undefined
来源：
The Journal of Antibiotics | 2008年 / 61卷
关键词：
benanomicin; biotransformation; P450; actinomycete;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Benanomicins were found as antifungal antibiotics from the culture of an actinomycete with potent antifungal activities in vitro and in vivo. We aimed to generate derivatives superior to benanomicin A by biotransformation using Escherichia coli constructed with bacterial P450 expression system. We found transformation of benanomicin A into two derivatives, 10-hydroxybenanomicin A and 11-O-demethylbenanomicin A by one of the P450-expressed strains which harbored a plasmid carrying a CYP105C1-homologous gene. Unexpectedly, the biotransformed compounds showed weak antifungal activities in vitro compared with those of benanomicin A.
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页码：394 / 399
页数：5
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