Destabilization of nucleophosmin mRNA by the HuR/KSRP complex is required for muscle fibre formation

被引：57

作者：

Cammas, Anne ^{[1
,2
]}

Sanchez, Brenda Janice ^{[1
]}

Lian, Xian Jin ^{[1
]}

Dormoy-Raclet, Virginie ^{[1
]}

van der Giessen, Kate ^{[1
]}

Lopez de Silanes, Isabel ^{[3
]}

Ma, Jennifer ^{[1
]}

Wilusz, Carol ^{[4
]}

Richardson, John ^{[5
]}

Gorospe, Myriam ^{[6
]}

Millevoi, Stefania ^{[2
]}

Giovarelli, Matteo ^{[7
]}

Gherzi, Roberto ^{[7
]}

Di Marco, Sergio ^{[1
]}

Gallouzi, Imed-Eddine ^{[1
]}

机构：

[1] McGill Univ, Dept Biochem, Goodman Canc Ctr, Montreal, PQ H3G 1Y6, Canada

[2] Ctr Rech Cancerol Toulouse, INSERM, UMR 1037, F-31432 Toulouse, France

[3] Spanish Natl Canc Res Ctr CNIO Telomeres & Telome, Mol Oncol Program, Madrid 28029, Spain

[4] Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA

[5] McGill Univ, Dept Neurol & Neurosurg, Montreal, PQ H3A 2B4, Canada

[6] NIA, NIH, Biomed Res Ctr, Baltimore, MD 21224 USA

[7] Ist Nazl Ric Cancro IST, Lab Gene Express Regulat, I-16132 Genoa, Italy

来源：

NATURE COMMUNICATIONS | 2014年 / 5卷

关键词：

AU-RICH ELEMENTS; BINDING-PROTEIN; GENE-EXPRESSION; HUR; DECAY; PHOSPHORYLATION; DIFFERENTIATION; IDENTIFICATION; STABILIZATION; CELLS;

D O I：

10.1038/ncomms5190

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

HuR promotes myogenesis by stabilizing the MyoD, myogenin and p21 mRNAs during the fusion of muscle cells to form myotubes. Here we show that HuR, via a novel mRNA destabilizing activity, promotes the early steps of myogenesis by reducing the expression of the cell cycle promoter nucleophosmin (NPM). Depletion of HuR stabilizes the NPM mRNA, increases NPM protein levels and inhibits myogenesis, while its overexpression elicits the opposite effects. NPM mRNA destabilization involves the association of HuR with the decay factor KSRP as well as the ribonuclease PARN and the exosome. The C terminus of HuR mediates the formation of the HuR-KSRP complex and is sufficient for maintaining a low level of the NPM mRNA as well as promoting the commitment of muscle cells to myogenesis. We therefore propose a model whereby the downregulation of the NPM mRNA, mediated by HuR, KSRP and its associated ribonucleases, is required for proper myogenesis.

引用

页数：16

共 60 条

[1] Posttranscriptional gene regulation by RNA-binding proteins during oxidative stress: implications for cellular senescence
Abdelmohsen, Kotb
Kuwano, Yuki
Kim, Hyeon Ho
Gorospe, Myriarn
[J]. BIOLOGICAL CHEMISTRY, 2008, 389 (03) : 243 - 255
[2] Posttranscriptional orchestration of an anti-apoptotic program by HuR
Abdelmohsen, Kotb
Lal, Ashish
Kim, Hyeon Ho
Gorospe, Myriam
[J]. CELL CYCLE, 2007, 6 (11) : 1288 - 1292
[3] miR-519 reduces cell proliferation by lowering RNA-binding protein HuR levels
Abdelmohsen, Kotb
Srikantan, Subramanya
Kuwano, Yuki
Gorospe, Myriam
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (51) : 20297 - 20302
[4] AU-rich elements and associated factors: are there unifying principles?
Barreau, C
Paillard, L
Osborne, HB
[J]. NUCLEIC ACIDS RESEARCH, 2005, 33 (22) : 7138 - 7150
[5] The cleavage of HuR interferes with its transportin-2-mediated nuclear import and promotes muscle fiber formation
Beauchamp, P.
Nassif, C.
Hillock, S.
van der Giessen, K.
von Roretz, C.
Jasmin, B. J.
Gallouzi, I-E
[J]. CELL DEATH AND DIFFERENTIATION, 2010, 17 (10) : 1588 - 1599
[6] Relief of microRNA-mediated translational repression in human cells subjected to stress
Bhattacharyya, Suvendra N.
Habermacher, Regula
Martine, Ursula
Closs, Ellen I.
Filipowicz, Witold
[J]. CELL, 2006, 125 (06) : 1111 - 1124
[7] HuR and mRNA stability
Brennan, CM
Steitz, JA
[J]. CELLULAR AND MOLECULAR LIFE SCIENCES, 2001, 58 (02) : 266 - 277
[8] p38-dependent phosphorylation of the mRNA decay-promoting factor KSRP controls the stability of select myogenic transcripts
Briata, P
Forcales, SV
Ponassi, M
Corte, G
Chen, CY
Karin, M
Puri, PL
Gherzi, R
[J]. MOLECULAR CELL, 2005, 20 (06) : 891 - 903
[9] Cytoplasmic relocalization of heterogeneous nuclear ribonucleoprotein A1 controls translation initiation of specific mRNAs
Cammas, Anne
Pileur, Frederic
Bonnal, Sophie
Lewis, Stephen M.
Leveque, Nicolas
Holcik, Martin
Vagner, Stephan
[J]. MOLECULAR BIOLOGY OF THE CELL, 2007, 18 (12) : 5048 - 5059
[10] HuR Uses AUF1 as a Cofactor To Promote p16INK4 mRNA Decay
Chang, Na
Yi, Jie
Guo, Gaier
Liu, Xinwen
Shang, Yongfeng
Tong, Tanjun
Cui, Qinghua
Zhan, Ming
Gorospe, Myriam
Wang, Wengong
[J]. MOLECULAR AND CELLULAR BIOLOGY, 2010, 30 (15) : 3875 - 3886

← 1 2 3 4 5 6 →