Cloning, tissue expression, and chromosomal assignment of human MRJ gene for a member of the DNAJ protein family

被引：0

作者：

Naohiko Seki

Atsushi Hattori

Akiko Hayashi

Sumie Kozuma

Nobuyuki Miyajima

T. Saito

机构：

[1] Genome Research Group,

[2] National Institute of Radiological Sciences,undefined

[3] 4-9-1 Anagawa,undefined

[4] Inage-ku,undefined

[5] Chiba 263-8555,undefined

[6] Japan Tel. +81-43-206-3135; Fax +81-43-251-9818 e-mail: t_saito@nirs.go.jp,undefined

[7] Biotechnology and Medical Engineering Field,undefined

[8] Aisin Cosmos,undefined

[9] Tokyo,undefined

[10] Japan,undefined

[11] Laboratory of Genome Information,undefined

[12] Kazusa DNA Research Institute,undefined

[13] Chiba,undefined

[14] Japan,undefined

来源：

Journal of Human Genetics | 1999年 / 44卷

关键词：

Key words DnaJ; MRJ; MSJ-1; J-domain; Chromosome 11q25;

D O I：

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中图分类号：

学科分类号：

摘要：

The DnaJ protein family consists of proteins with a highly conserved amino acid stretch called the "J - domain". A cDNA clone encoding a new protein with a J-domain was isolated from a human fetal brain cDNA library. This new member of the DnaJ family of 241 amino acid residues showed 94% identity with mouse Mrj (accession number, AF035962) and 71% identity with mouse Msj-1 (accession number, U95607) along its entire sequence. Reverse transcription - coupled polymerase chain reaction (RT-PCR) analysis showed the messenger RNA was ubiquitously expressed in various human tissues. The chromosomal location of the gene was determined by PCR-based analyses with both a human/rodent monochromosomal hybrid cell panel and a radiation hybrid panel to map on chromosome 11q25 region.

引用

页码：185 / 189

页数：4