Quantifying CD4 receptor protein in two human CD4+ lymphocyte preparations for quantitative flow cytometry

被引：1
|
作者：
Meiyao Wang
Martin Misakian
Hua-Jun He
Peter Bajcsy
Fatima Abbasi
Jeffrey M Davis
Kenneth D Cole
Illarion V Turko
Lili Wang
机构：
[1] University of Maryland,Institute for Bioscience and Biotechnology Research
[2] National Institute of Standards and Technology (NIST),Biomolecular Measurement Division
[3] NIST,Quantum Measurements Division
[4] NIST,Biosystems and Biomaterials Division
[5] NIST,Software and Systems Division
[6] CBER FDA,Laboratory of Stem Cell Biology, Cellular and Tissue Therapy Branch, Division of Cell and Gene Therapies
[7] NIST,Materials Measurement Science Division
来源：
Clinical Proteomics | 2014年 / 11卷
关键词：
Cryopreserved peripheral blood mononuclear cells (PBMC); Lyophilized Cyto-Trol™; CD4 receptor density; Flow cytometry; Multiple reaction monitoring (MRM) mass spectrometry (MS); Scanning electron microscopy (SEM); Microvilli; Cell surface area;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
引用
收藏
相关论文
共 50 条
  • [21] Three-color flow cytometry detection of virus-specific CD4+ and CD8+ T cells in the cat
    de Groot-Mijnes, JDF
    van der Most, RG
    van Dun, JM
    te Lintelo, EG
    Schuurman, NMP
    Egberink, HF
    de Groot, RJ
    JOURNAL OF IMMUNOLOGICAL METHODS, 2004, 285 (01) : 41 - 54
  • [22] Absolute and percent CD4+ T-cell enumeration by flow cytometry using capillary blood
    Sitoe, Nadia
    Luecke, Ellen
    Tembe, Nelson
    Matavele, Raquel
    Cumbane, Victoria
    Macassa, Eugenia
    Vaz, Paula
    Sheppard, Haynes
    Jani, Ilesh V.
    JOURNAL OF IMMUNOLOGICAL METHODS, 2011, 372 (1-2) : 1 - 6
  • [23] Flow cytometry absolute and relative values of CD3CD4 cells obtained by two different techniques
    Galassi, N
    Riera, N
    Saracco, M
    Aixala, M
    De Bracco, MME
    ACTA BIOQUIMICA CLINICA LATINOAMERICANA, 1999, 33 (03): : 331 - 337
  • [24] QUANTITATIVE-DETERMINATION OF CD4/CD8 MOLECULES BY A CELL MARKER ELISA
    FRANKE, L
    NUGEL, E
    DOCKE, WD
    PORSTMANN, T
    CLINICAL CHEMISTRY, 1994, 40 (01) : 38 - 42
  • [25] CD4 estimating reagents in dry format are compatible with conventional flow cytometer; FACSCalibur for estimation of absolute CD4 count & percentages
    Thakar, Madhuri
    Mahajan, Bharati
    Joshi, Trupti
    Sane, Suvarna
    Paranjape, Ramesh
    INDIAN JOURNAL OF MEDICAL RESEARCH, 2013, 137 : 346 - 355
  • [26] Automated cell count in flow cytometry -: A valuable tool to assess CD4 absolute levels in peripheral blood
    Béné, MC
    Sarda, MNK
    El Kaissouni, J
    Kennel, AD
    Molé, C
    Kohler, C
    Faure, GC
    AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1998, 110 (03) : 321 - 326
  • [27] Monitoring of Cytomegalovirus-Specific CD4+ and CD8+ T Cell Responses by Cytokine Flow Cytometry in Renal Transplant Recipients
    Kilinckaya Dogan, Hafize
    Mutlu, Esvet
    Koksoy, Sadi
    Yilmaz, Vural T.
    Kocak, Huseyin
    Colak, Dilek
    Mutlu, Derya
    Gunseren, Filiz
    Dinckan, Ayhan
    Aliosmanoglu, Ibrahim
    Suleymanlar, Gultekin
    Gultekin, Meral
    MIKROBIYOLOJI BULTENI, 2016, 50 (02): : 224 - 235
  • [28] Use of the multiparametric panel CD3/CD4/CD8/CD7/CD26/CD158k in the detection and use of flow cytometry of Sezary cells
    Callet, Jonas
    Latger-Cannard, Veronique
    Gerard, Delphine
    Salignac, Sylvain
    Granel-Brocard, Florence
    Campidelli, Arnaud
    Bursztejn, Anne-Claire
    Broseus, Julien
    Vial, Jean-Philippe
    Lesesve, Jean-Francois
    ANNALES DE BIOLOGIE CLINIQUE, 2021, 79 (03) : 233 - 240
  • [29] Comparison of two flow cytometric methods enumerating CD4 T cells and CD8 T cells
    Storek, J
    Dawson, MA
    Maloney, DG
    CYTOMETRY, 1998, 33 (01): : 76 - 82
  • [30] TRIPLE IMMUNOFLUORESCENCE FLOW-CYTOMETRY, USING WHOLE-BLOOD, OF CD4+ AND CD8+ LYMPHOCYTES EXPRESSING CD45RO AND CD45RA
    GRUBER, R
    REITER, C
    RIETHMULLER, G
    JOURNAL OF IMMUNOLOGICAL METHODS, 1993, 163 (02) : 173 - 179
12345