Quantifying CD4 receptor protein in two human CD4+ lymphocyte preparations for quantitative flow cytometry

被引:1
作者
Meiyao Wang
Martin Misakian
Hua-Jun He
Peter Bajcsy
Fatima Abbasi
Jeffrey M Davis
Kenneth D Cole
Illarion V Turko
Lili Wang
机构
[1] University of Maryland,Institute for Bioscience and Biotechnology Research
[2] National Institute of Standards and Technology (NIST),Biomolecular Measurement Division
[3] NIST,Quantum Measurements Division
[4] NIST,Biosystems and Biomaterials Division
[5] NIST,Software and Systems Division
[6] CBER FDA,Laboratory of Stem Cell Biology, Cellular and Tissue Therapy Branch, Division of Cell and Gene Therapies
[7] NIST,Materials Measurement Science Division
来源
Clinical Proteomics | 2014年 / 11卷
关键词
Cryopreserved peripheral blood mononuclear cells (PBMC); Lyophilized Cyto-Trol™; CD4 receptor density; Flow cytometry; Multiple reaction monitoring (MRM) mass spectrometry (MS); Scanning electron microscopy (SEM); Microvilli; Cell surface area;
D O I
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学科分类号
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[1]  
Poncelet P(1991)Surface CD4 density remains constant on lymphocytes of HIV-infected patients in the progression of disease Res Immunol 142 291-298
[2]  
Poinas G(1998)Quantitation of CD38 activation antigen expression on CD8+ T cells in HIV-1 infection using CD4 expression on CD4+ T lymphocytes as a biological calibrator Cytometry 33 123-132
[3]  
Corbeau P(1998)Determination of CD4 antigen density on cells: role of antibody valency, avidity, clones, and conjugation Cytometry 33 197-205
[4]  
Devaux C(2008)Toward quantitative fluorescence measurements with multicolor flow cytometry Cytometry A 73A 279-288
[5]  
Tubiana N(2012)Human CD4+ lymphocytes for antigen quantification: characterization using conventional flow cytometry and mass cytometry Cytometry A 81A 567-575
[6]  
Muloko N(2013)Quantifying CD4 receptor density on human T lymphocytes using multiple reaction monitoring mass spectrometry Anal Chem 85 1773-1777
[7]  
Tamalet C(2001)CCR5, CXCR4, and CD4 are clustered and closely apposed on microvilli of human macrophages and T cells J Virol 75 3779-3790
[8]  
Chermann JC(2003)Localization of CD4 and CCR5 in living cells J Virol 77 4985-4991
[9]  
Kourilsky F(2006)Differential role of lipid rafts in the functions of CD4+ and CD8+ human T lymphocytes with aging Cell Signal 8 1017-1030
[10]  
Sampol J(2000)Lyophilization and development of solid protein pharmaceuticals Int J Pharm 203 1-60