Shoot multiplication in cultures of mature Alnus glutinosa

被引:0
|
作者
S. Lall
Z. Mandegaran
A. V. Roberts
机构
[1] University of East London,School of Health and Bioscience
来源
Plant Cell, Tissue and Organ Culture | 2005年 / 83卷
关键词
1-; - naphthylphthalamic acid (NPA); 2,3,5-triiodobenzoic acid (TIBA); auxin transport inhibitors; mature trees; somatic embryos;
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摘要
Shoot cultures were initiated from mature trees of Alnus glutinosa. On medium containing 1–5 μM 6-benzylamino purine (BAP), the shoots elongated without branching, formed heavy callus at the base of the stems and readily formed roots. The possibility that these characteristics could be attributed to the strong influence of endogenous auxin was tested on media that contained two auxin transport inhibitors, 1-N- naphthylphthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA), at concentrations of 0.1–3 μM, in combination with 2 μM BAP. On these media, shoots produced numerous branches, less callus and no roots. After 30 weeks (five subcultures) on this medium, leaves were smaller and showed signs of vitrification. These problems were resolved without detriment to shoot proliferation, by reverting to medium without NPA or TIBA. Shoots rooted readily after transfer to medium without growth regulators and were successfully acclimatised after transfer to soil.
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页码:347 / 350
页数:3
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