Isolation and characterization of tetranucleotide microsatellite markers for Pinus merkusii

被引：0
|
作者：
D. V. Thao
A. Y. P. B. C. Widyatmoko
L. Guan
E. Gotoh
A. Watanabe
S. Shiraishi
机构：
[1] Kyushu University,Graduate School of Bioresource and Bioenvironmental Sciences
[2] Centre for Forest Biotechnology and Tree Improvement,Laboratory of Silviculture, Faculty of Agriculture
[3] Hubei Provincial Forest Tree Breeding Centre,undefined
[4] Kyushu University,undefined
来源：
Conservation Genetics Resources | 2013年 / 5卷
关键词：
Tetranucleotide microsatellite marker; Dual-suppression PCR;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Pinus merkusii is an important tree native to Southeast Asia. Thirteen tetranucleotide microsatellite makers were developed from this species using a dual-suppression polymerase chain reaction technique. Of these markers, nine loci were polymorphic. The mean number of alleles per locus was 3.18 (range 2–5) and mean expected heterozygosities were 0.525 (range 0.384–0.712). These microsatellite markers will be available to assess the genetic diversity, mating patterns and population structure which will facilitate conservation and management strategies.
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页码：433 / 436
页数：3
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