Genetic map construction and QTL analysis of nitrogen use efficiency in spinach (Spinacia oleracea L.)

被引：0
作者：
Rafael Chan-Navarrete
Oene Dolstra
Martijn van Kaauwen
Edith T. Lammerts van Bueren
C. Gerard van der Linden
机构：
[1] Wageningen University and Research Centre,Wageningen UR Plant Breeding
[2] The C.T. de Wit Graduate School Production Ecology - Resource Conservation (PE&RC),undefined
来源：
Euphytica | 2016年 / 208卷
关键词：
Nitrogen use efficiency (NUE); Quantitative trait locus (QTL); Genetic map;
D O I：
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中图分类号：
学科分类号：
摘要：
Cultivation of spinach requires high amounts of nitrogen (N), which puts a strain on the environment. A sustainable solution to this problem is to breed for crops with higher N use efficiency (NUE). The aim of this study was to provide tools for molecular breeding and to elucidate the genetic variation of factors contributing to NUE in spinach. A cross was made between two F1 hybrid cultivars contrasting in NUE. Several F1 progeny were self-pollinated and based on evaluation of the F2 generation, a mapping F2 population (335 individuals) of a single F1 was selected. SNP markers for the genetic map were discovered by RNA sequencing of the two parent cultivars, and 283 SNP markers were used to produce a genetic map comprising of six linkage groups (P01–P06), ranging in size from 46 to 116 cM. NUE related traits were determined for a set of F2:3 families grown under low and high N conditions in a hydroponics system under an Ingestad N-addition model. Interval mapping analysis detected 39 trait-specific QTLs, with several QTLs accumulating on P01 and P02 of the linkage map. The QTLs and in particular the P01 and P02 regions provide potential targets for the improvement of NUE in spinach.
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页码：621 / 636
页数：15
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