Biosynthesis, Characterization, and Evaluation of the Cytotoxic Effects of Biologically Synthesized Silver Nanoparticles from Cyperus conglomeratus Root Extracts on Breast Cancer Cell Line MCF-7

被引:0
|
作者
Amani Ghassan Al-Nuairi
Kareem A. Mosa
Mohammad G. Mohammad
Ali El-Keblawy
Sameh Soliman
Hussain Alawadhi
机构
[1] University of Sharjah,Department of Applied Biology, College of Sciences
[2] Al-Azhar University,Department of Biotechnology, Faculty of Agriculture
[3] University of Sharjah,Department of Medical Laboratory Sciences, Collage of Health Sciences
[4] University of Sharjah,Iron Biology research group, Sharjah Institute of Medical Research
[5] University of Sharjah,College of Pharmacy
[6] University of Sharjah,Infectous Disease Research Group, Sharjah Institute of Medical Research
[7] University of Sharjah,Center for Advanced Materials Research, Research Institute of Sciences and Engineering
来源
Biological Trace Element Research | 2020年 / 194卷
关键词
Silver nanoparticles ; Anticancer ; Apoptosis;
D O I
暂无
中图分类号
学科分类号
摘要
Over recent years, metal nanoparticles have largely been investigated due to their potential activities. This study focused on synthesizing silver nanoparticles (AgNPs) using the desert plant Cyperus conglomeratus, which is the most abundant species on the sand dunes in the UAE, and their anticancer activity. The synthesized AgNPs were characterized using UV-visible spectra, X-ray diffraction, energy dispersive X-ray spectroscopy, fourier transform infrared spectroscopy, dynamic light scattering, and scanning electron microscope. The results showed that the AgNPs are monodispersed and mostly spherical in shape. The cytotoxicity effects were investigated against breast cancer cells MCF-7 and normal fibroblast using MTT assay which showed selective cytotoxicity against MCF-7 with an IC50 at 5 μg/mL but not fibroblast. Moreover, the apoptotic effects were confirmed using annexin V-FITC-PI double staining kit and real-time PCR for apoptotic genes. Therefore, our results revealed potential anticancer applications of the C. conglomeratus biosynthesized silver nanoparticles.
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收藏
页码:560 / 569
页数:9
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