A novel lectin with highly potent antiproliferative and HIV-1 reverse transcriptase inhibitory activities from cicada (Cicada flammata)

被引：0
作者：
Xiu Juan Ye
Tzi Bun Ng
机构：
[1] The Chinese University of Hong Kong,School of Biomedical Sciences, Faculty of Medicine
来源：
Applied Microbiology and Biotechnology | 2010年 / 86卷
关键词：
Cicada; Lectin; Antiproliferative; HIV-1 reverse transcriptase;
D O I：
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学科分类号：
摘要：
A dimeric lectin with a molecular weight of 60 kDa and high hemagglutinating activity was isolated from dried cicadas. It was adsorbed on Q-Sepharose and unadsorbed on Affi-Gel Blue gel. Its hemagglutinating activity was stable up to 55 °C and between pH 2 and 13. The activity was inhibited by glucuronic acid and raffinose, K+ ions, and Mg2+ ions. Cicada lectin potently inhibited proliferation of HepG2 hepatoma and MCF 7 breast cancer cells, with an IC50 value of 0.76 and 0.49 µM, respectively. It potently inhibited HIV-1 reverse transcriptase activity with an IC50 of 0.36 μM but was devoid of mitogenic activity on spleen cells. Its N-terminal sequence exhibited slight similarity to a conserved hypothetical protein from Culex quinquefasciatus and a gene product from transcript GH19834-RA of Drosophila grimshawi, but there was no resemblance to lectins from other insects, including Drosophila, Sarcophaga, Glossina, and Aedes species.
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页码：1409 / 1418
页数：9
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