Taking snapshots of photosynthetic water oxidation using femtosecond X-ray diffraction and spectroscopy

被引：0

作者：

Jan Kern

Rosalie Tran

Roberto Alonso-Mori

Sergey Koroidov

Nathaniel Echols

Johan Hattne

Mohamed Ibrahim

Sheraz Gul

Hartawan Laksmono

Raymond G. Sierra

Richard J. Gildea

Guangye Han

Julia Hellmich

Benedikt Lassalle-Kaiser

Ruchira Chatterjee

Aaron S. Brewster

Claudiu A. Stan

Carina Glöckner

Alyssa Lampe

Dörte DiFiore

Despina Milathianaki

Alan R. Fry

M. Marvin Seibert

Jason E. Koglin

Erik Gallo

Jens Uhlig

Dimosthenis Sokaras

Tsu-Chien Weng

Petrus H. Zwart

David E. Skinner

Michael J. Bogan

Marc Messerschmidt

Pieter Glatzel

Garth J. Williams

Sébastien Boutet

Paul D. Adams

Athina Zouni

Johannes Messinger

Nicholas K. Sauter

Uwe Bergmann

Junko Yano

Vittal K. Yachandra

机构：

[1] Lawrence Berkeley National Laboratory,Physical Biosciences Division

[2] LCLS,undefined

[3] SLAC National Accelerator Laboratory,undefined

[4] Institutionen för Kemi,undefined

[5] Kemiskt Biologiskt Centrum,undefined

[6] Umeå Universitet,undefined

[7] Institut für Biologie,undefined

[8] Humboldt-Universität zu Berlin,undefined

[9] Max-Volmer-Laboratorium für Biophysikalische Chemie,undefined

[10] Technische Universität,undefined

[11] PULSE Institute,undefined

[12] SLAC National Accelerator Laboratory,undefined

[13] European Synchrotron Radiation Facility,undefined

[14] SSRL,undefined

[15] SLAC National Accelerator Laboratory,undefined

[16] National Energy Research Scientific Computing Center,undefined

[17] Lawrence Berkeley National Laboratory,undefined

[18] Present address: Diamond Light Source,undefined

[19] Harwell Science and Innovation Campus,undefined

[20] Didcot,undefined

[21] Oxfordshire OX11 0DE,undefined

[22] UK,undefined

[23] Present address: Synchrotron SOLEIL,undefined

[24] F-91192 Gif-Sur-Yvette,undefined

[25] France,undefined

[26] Present address: Department of Cell and Molecular Biology,undefined

[27] Uppsala Universitet,undefined

[28] 751 24 Uppsala,undefined

[29] Sweden,undefined

来源：

Nature Communications | / 5卷

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摘要：

The dioxygen we breathe is formed by light-induced oxidation of water in photosystem II. O2 formation takes place at a catalytic manganese cluster within milliseconds after the photosystem II reaction centre is excited by three single-turnover flashes. Here we present combined X-ray emission spectra and diffraction data of 2-flash (2F) and 3-flash (3F) photosystem II samples, and of a transient 3F’ state (250 μs after the third flash), collected under functional conditions using an X-ray free electron laser. The spectra show that the initial O–O bond formation, coupled to Mn reduction, does not yet occur within 250 μs after the third flash. Diffraction data of all states studied exhibit an anomalous scattering signal from Mn but show no significant structural changes at the present resolution of 4.5 Å. This study represents the initial frames in a molecular movie of the structural changes during the catalytic reaction in photosystem II.

引用

共 50 条

[1] Taking snapshots of photosynthetic water oxidation using femtosecond X-ray diffraction and spectroscopy
Kern, Jan
Tran, Rosalie
Alonso-Mori, Roberto
Koroidov, Sergey
Echols, Nathaniel
Hattne, Johan
Ibrahim, Mohamed
Gul, Sheraz
Laksmono, Hartawan
Sierra, Raymond G.
Gildea, Richard J.
Han, Guangye
Hellmich, Julia
Lassalle-Kaiser, Benedikt
Chatterjee, Ruchira
Brewster, Aaron S.
Stan, Claudiu A.
Gloeckner, Carina
Lampe, Alyssa
DiFiore, Doertee
Milathianaki, Despina
Fry, Alan R.
Seibert, M. Marvin
Koglin, Jason E.
Gallo, Erik
Uhlig, Jens
Sokaras, Dimosthenis
Weng, Tsu-Chien
Zwart, Petrus H.
Skinner, David E.
Bogan, Michael J.
Messerschmidt, Marc
Glatzel, Pieter
Williams, Garth J.
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Adams, Paul D.
Zouni, Athina
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