Serum IL-4, IL-12 and TNF-alpha in malaria: A comparative study associating cytokine responses with severity of disease from the Coastal Districts of Odisha

被引：20

作者：

Sarangi A. ^{[1
]}

Mohapatra P.C. ^{[1
]}

Dalai R.K. ^{[2
]}

Sarangi A.K. ^{[3
]}

机构：

[1] Department of Biochemistry, S.C.B. Medical College, Cuttack, Odisha

[2] Department of Medicine, S.C.B. Medical College, Cuttack, Odisha

[3] School of Biotech Sciences, Trident Academy of Creative Technology, Bhubaneswar, Odisha

来源：

Journal of Parasitic Diseases | 2014年 / 38卷 / 2期

关键词：

Clinical severity; Cytokines; IL-12; IL-4; Malaria; TNF-alpha;

D O I：

10.1007/s12639-013-0237-1

中图分类号：

学科分类号：

摘要：

We investigated the role of IL-4, IL-12 and TNF-alpha in clinically well-defined groups of Plasmodium falciparum and vivax (Pf & Pv) infected patients belonging to Group I (++), Group II (+++) and Group III (++++). On the basis of hematological parameters, hyperparasitaemia, and evidence of neurological involvement, three different levels of severity were selected attributing a score from Group I (++) to Group III (++++). In each group 16 patients each of P. falciparum and P. vivax malaria were studied. As a control group for cytokine determination 30 healthy volunteers were included in the study. Serum samples were analyzed for IL-12, IL-4 and TNF-alpha using (ELISA) obtained commercially (Ray Biotech). Hb levels of Pf and Pv patients were 8 ± 1.94, 7.6 ± 1.64 g/dl and 3.6 ± 1.23 and 10.1 ± 1.21, 9.4 ± 1.43 and 7.1 ± 0.98 g/dl. Serum iron levels of Pf and Pv patients were 85.86 ± 0.86, 81.10 ± 0.70 and 70.1 ± 0.73 and 99.47 ± 0.85, 96.67 ± 1.13 and 91.7 ± 2.65 mg/dl. TNF-alpha levels of Pf and Pv patients were 155 ± 23.66, 307.5 ± 111.87 and 955 ± 261.32 and 72 ± 9.93, 140.88 ± 23.11 and 469.37 ± 416.99 pg/ml. IL-12 levels of Pf and Pv patients were 117.5 ± 8.16, 160.63 ± 20.81 and 293.13 ± 94.64 and 75.7 ± 9.25, 112.9 ± 12.05 and 200 ± 53.78 pg/ml. IL-4 levels of Pf and Pv patients were 3.7 ± 0.11, 3.2 ± 0.13 and 2.3 ± 0.63 and 5.33 ± 1.08, 4.8 ± 0.16 and 3.9 ± 0.48 pg/ml. In the control group the values of TNF-alpha, IL-12 and IL-4 were 42.9 ± 13.5, 49.8 ± 11.59 and 6.06 ± 1.32 pg/ml respectively. Cytokines and poor oxygen delivery should not be viewed as alternative theories of malarial disease pathophysiology instead poor oxygen delivery is one of the consequences of excessive release of inflammatory cytokines which is further augmented by the present work. © 2013 Indian Society for Parasitology.

引用

页码：143 / 147

页数：4

共 17 条

[1]

Andersson U., Wang H.C., Palmblad K., Aveberger A.C., Et al., High mobility group 1 protein (HMG-1) stimulates proinflammatory cytokine synthesis in human monocytes, J Exp Med, 192, pp. 565-570, (2000)

[2]

Clark A.I., Budd C.A., Alleva M.L., Cowden B.W., Human malarial disease. A consequence of inflammatory cytokine release, Malaria J, 5, pp. 85-117, (2006)

[3]

Crutcher J.M., Stevenson M., Sedegah M., Hoffman S.L., Interleukin 12 and malaria, Res Immunol, 146, pp. 552-559, (1995)

[4]

Day N.P., Hien T.T., Schollaardt T., Loc P.P., Et al., The prognostic and pathophysiology role of pro- and anti-inflammatory cytokines in severe malaria, J Infect Dis, 180, pp. 1288-1297, (1999)

[5]

Gogos C.A., Drosou E., Bassaris H.P., Skoutelis A., Pro-versus anti-inflammatory cytokine profile in patients with severe sepsis: a marker of prognosis and future therapeutic options, J Infect Dis, 181, pp. 176-180, (2000)

[6]

Grau G.E., Taylor T.E., Molyneux M.E., Wirima J.J., Vassalli P., Hommel M., Lambert P.H., Tumor necrosis factor and diseases severity in children with falciparum malaria, N Engl J Med, 320, pp. 1586-1591, (1989)

[7]

Kumaratilake M.L., Ferrante A., Rzepczyk M.C., Tumor necrosis factor enhances neutrophil-mediated killing of Plasmodium falciparum, Infect Immun, 58, pp. 788-793, (1990)

[8]

Luty F.J.A., Perkins P.J., Lell B., Ott S.R., Et al., Low interleukin 12 activity in severe Plasmodium falciparum malaria, Infect Immun, 68, pp. 3909-3915, (2000)

[9]

Lyke K.E., Burges R., Cissoko Y., Et al., Serum levels of the proinflammatory cytokines interleukin-1 beta (IL-1beta), IL-6, IL-8, IL-10, tumor necrosis factor alpha and IL-12 (p70) in Malian children with severe Plasmodium falciparum malaria and matched uncomplicated malaria or healthy controls, Infect Immun, 72, pp. 5630-5637, (2004)

[10]

Malaguarnera L., Musumeci S., The immune response to Plasmodium falciparum malaria (review), Lancet Infect Dis, 2, pp. 472-478, (2002)

← 1 2 →