Adventitious shoot regeneration and appearance of sports in several azalea cultivars

被引：0
作者：
G. Samyn
S. De Schepper
E. Van Bockstaele
机构：
[1] CLO-Gent,Department Plant Genetics and Breeding
[2] Ghent University,Department of Plant Production
来源：
Plant Cell, Tissue and Organ Culture | 2002年 / 70卷
关键词：
periclinal chimeras; petioles; picotee; recalcitrance; somaclonal variation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Shoots can be regenerated from leaf explants of most azalea (Rhododendron simsii) cultivars using a two-step protocol with a callus induction and a regeneration phase. This procedure is unsuccessful for a few cultivars, because of excessive callus production. In most such cases, shoot regeneration could be obtained in one step, starting from petioles as explants. Flower colour variations (the induction of sports) were frequently observed after regeneration. This phenomenon offers prospects for the enlargement of the commercial assortment but can also be used for the study of gene regulation processes in flower colour variegation.
引用
收藏
页码：223 / 227
页数：4
相关论文
共 22 条
  • [1] Anderson WC(1984)A revised tissue culture medium for shoot multiplication of J. Am. Soc. Hortic. Sci. 109 343-347
  • [2] Chittenden RJ(1927)Vegetative segregetion Bibl. Genet. 3 355-442
  • [3] De Schepper S(2001)Somatic polyploidy and its consequences for flower coloration and flower morphology in azalea Plant Cell Rep. 20 583-590
  • [4] Leus L(1997)Chimeras and variegation: patterns of deceit HortScience 32 773-784
  • [5] Mertens M(1994) regeneration of evergreen azalea from leaves Regeneration and somaclonal variation of Rhododendron simsii offer prospects to the obtainment of commercially valuable sports 59/4b 2495-2497
  • [6] Debergh P(1996)A revised medium for rapid growth and bioassays with tobacco tissue cultures Plant Cell Tiss. Org. Cult. 45 231-236
  • [7] Van Bockstaele E(1962)Effects of growth regulators and incubation period on Physiol. Plant. 15 473-497
  • [8] De Loose M(1999) regeneration of adventitious shoots from gerbera petioles Plant Cell Tiss. Org. Cult. 59 95-102
  • [9] Marcotrigiano M(undefined)undefined undefined undefined undefined-undefined
  • [10] Mertens M(undefined)undefined undefined undefined undefined-undefined
123