Media optimization using Box Behnken design for enhanced production of biomass, beta-carotene and lipid from Dunaliella salina

被引：11

作者：

Ahuja S. ^{[1
]}

Roy A. ^{[1
]}

Kumar L. ^{[1
]}

Bharadvaja N. ^{[1
]}

机构：

[1] Plant Biotechnology Laboratory, Department of Biotechnology, Delhi Technological University, New Delhi

来源：

Vegetos | 2020年 / 33卷 / 1期

关键词：

Beta-carotene; Box Behnken design; Lipids; Response surface methodology;

D O I：

10.1007/s42535-019-00079-4

中图分类号：

学科分类号：

摘要：

Dunaliella salina a halotolerant microalga is well known for a high fatty acid and beta-carotene content, which makes it a potent source at a commercial level. The current study focuses on optimizing commercially known media using Box Behnken design to attain higher yields of biomass, beta-carotene, and lipids simultaneously. The optimal medium conditions as per response surface methodology were glucose, potassium nitrate, sodium chloride at a concentration of 13.23 g/L, 3.145 g/L and 35.6 g/L, respectively while maintaining the concentration of other nutrients unchanged. Maximum yield of biomass, beta-carotene and lipid productivity attained experimentally using the optimized media was 1.24 g/L, 6.07 mg/g and 20.7 mg/L/day, respectively than their original values i.e., 0.571 g/L of biomass, 4.18 mg/g of beta-carotene and 13.2 mg/L/day of lipid content. Biomass yield was increased by 2.17 folds, beta-carotene and lipid were increased by 1.45 folds and 1.56 folds, respectively. © 2019, Society for Plant Research.

引用

页码：31 / 39

页数：8

共 32 条

[1] Ahmed R.A., He M., Aftab R.A., Zheng S., Nagi M., Bakri R., Wang C., Bioenergy application of Dunaliella salina SA 134 grown at various salinity levels for lipid production, Sci Rep, 7, 1, (2017)
[2] Azma M., Mohamed M.S., Mohamad R., Rahim R.A., Ariff A.B., Improvement of medium composition for heterotrophic cultivation of green microalgae, Tetraselmis suecica, using response surface methodology, Biochem Eng J, 53, 2, pp. 187-195, (2011)
[3] Bonnefond H., Moelants N., Talec A., Mayzaud P., Bernard O., Sciandra A., Coupling and uncoupling of triglyceride and beta-carotene production by Dunaliella salina under nitrogen limitation and starvation, Biotechnol Biofuels, 10, (2017)
[4] Cheirsilp B., Torpee S., Enhanced growth and lipid production of microalgae under mixotrophic culture condition: effect of light intensity, glucose concentration and fed-batch cultivation, Bioresour Technol, 110, pp. 510-516, (2012)
[5] Chen H., Jiang J.G., Wu G.H., Effects of salinity changes on the growth of Dunaliella salina and its isozyme activities of glycerol-3-phosphate dehydrogenase, J Agric Food Chem, 57, 14, pp. 6178-6182, (2009)
[6] Chen M., Tang H., Ma H., Holland T.C., Ng K.Y., Salley S.O., Effect of nutrients on growth and lipid accumulation in the green algae Dunaliella tertiolecta, Bioresour Technol, 102, 2, pp. 1649-1655, (2011)
[7] Converti A., Casazza A.A., Ortiz E.Y., Perego P., Del Borghi M., Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production, Chem Eng Process, 48, pp. 1146-1151, (2009)
[8] Del Campo J.A., Garcia-Gonzalez M., Guerrero M.G., Outdoor cultivation of microalgae for carotenoid production: current state and perspectives, Appl Microbiol Biotechnol, 74, pp. 1163-1174, (2007)
[9] Dominguez Teles I., The Fatter the Better: Selecting Microalgae Cells for Outdoor Lipid Production, (2016)
[10] Fakhry E.M., El Maghraby D.M., Lipid accumulation in response to nitrogen limitation and variation of temperature in Nannochloropsis salina, Botanical studies, 56, 1, (2015)

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