Effect of culture conditions on monoclonal antibody production from genetically modified tobacco suspension cultures

被引：0
作者：
Liu F. [1 ]
Lee J.M. [1 ]
机构：
[1] Department of Chemical Engineering, Washington State University, Pullman
来源：
Biotechnology and Bioprocess Engineering | 1999年 / 4卷 / 4期
关键词：
Glutamine addition; Inoculum age; Monoclonal antibody; Oxygen supply; Tobacco suspension cultures;
D O I：
10.1007/BF02933749
中图分类号：
学科分类号：
摘要：
Oxygen supply and inoculum age were found to affect the production of the heavy chain monoclonal antibody (HC MAb) from genetically modified tobacco suspension cultures. The increase of oxygen supply increased both cell growth and HC MAb production. Furthermore, the increased aeration and mixing improved the production of HC MAb based on the unit amount of cells or total soluble proteins. This indicated that the increased aeration improved the production and secretion of HC MAb more than other cell components. HC MAb production and cell growth also improved when batch cultures were inoculated with actively dividing cells (5-day old) rather than the fully-grown cells (7- or 10-day old cells) that are commonly used for subcultures. The addition of glutamine to the medium also improved cell growth and HC MAb production.
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页码：259 / 263
页数：4
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