Solid lipid nanoparticles loading adefovir dipivoxil for antiviral therapy

被引：0
作者：
Zhang Xing-guo
Miao Jing
Li Min-wei
Jiang Sai-ping
Hu Fu-qiang
Du Yong-zhong
机构：
[1] Zhejiang University,Department of Pharmacy, the First Affiliated Hospital, School of Medicine
[2] Zhejiang University,College of Pharmaceutical Sciences
[3] Zhejiang University,State Key Lab for Diagnosis and Treatment of Infectious Diseases
来源：
Journal of Zhejiang University SCIENCE B | 2008年 / 9卷
关键词：
Adefovir dipivoxil (ADV); Solid lipid nanoparticles (SLN); Octadecylamine-fluorescein isothiocynate (ODA-FITC); Hepatitis B virus (HBV); R512.6; 2; R943; R978.7;
D O I：
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学科分类号：
摘要：
Herein, solid lipid nanoparticles (SLN) were proposed as a new drug delivery system for adefovir dipivoxil (ADV). The octadecylamine-fluorescein isothiocynate (ODA-FITC) was synthesized and used as a fluorescence maker to be incorporated into SLN to investigate the time-dependent cellular uptake of SLN by HepG2.2.15. The SLN of monostearin with ODA-FITC or ADV were prepared by solvent diffusion method in an aqueous system. About 15 wt% drug entrapment efficiency (EE) and 3 wt% drug loading (DL) could be reached in SLN loading ADV. Comparing with free ADV, the inhibitory effects of ADV loaded in SLN on hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg) and hepatitis B virus (HBV) DNA levels in vitro were significantly enhanced.
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页码：506 / 510
页数：4
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